دورية أكاديمية
أعرض في EDS

Antioxidant and Anti-Inflammatory Effects of Chaenomeles sinensis Leaf Extracts on LPS-Stimulated RAW 264.7 Cells.

التفاصيل البيبلوغرافية
العنوان: Antioxidant and Anti-Inflammatory Effects of Chaenomeles sinensis Leaf Extracts on LPS-Stimulated RAW 264.7 Cells.
المؤلفون: Han YK; Korea Food Research Institute, Seongnam 13539, Korea. thwk00@naver.com., Kim YS; Department of Biotechnology, Konkuk University, Chungju 27478, Korea. kimyonsuk@kku.ac.kr., Natarajan SB; Department of Biotechnology, Konkuk University, Chungju 27478, Korea. nsboopathy@gmail.com., Kim WS; Department of Pharmaceutical Engineering, Silla University, Busan 46958, Korea. wskim@silla.ac.kr., Hwang JW; Department of Biotechnology, Konkuk University, Chungju 27478, Korea. croucard@kku.ac.kr., Jeon NJ; Department of Biotechnology, Konkuk University, Chungju 27478, Korea. hidingkking@kku.ac.kr., Jeong JH; Department of Food Science and Technology, Korea National University of Transportation, Jeungpyeong 27909, Korea. jhjeong@ut.ac.kr., Moon SH; Korea Nokyong Research Center, Konkuk University, Chungju 27478, Korea. moon0204@kku.ac.kr., Jeon BT; Korea Nokyong Research Center, Konkuk University, Chungju 27478, Korea. hannokwon@kku.ac.kr., Park PJ; Department of Biotechnology, Konkuk University, Chungju 27478, Korea. parkpj@kku.ac.kr.; Korea Nokyong Research Center, Konkuk University, Chungju 27478, Korea. parkpj@kku.ac.kr.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2016 Mar 28; Vol. 21 (4), pp. 422. Date of Electronic Publication: 2016 Mar 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Anti-Inflammatory Agents/*pharmacology , Antioxidants/*pharmacology , Inflammation/*drug therapy , Plant Extracts/*pharmacology , Rosaceae/*chemistry, Animals ; Anti-Inflammatory Agents/chemistry ; Antioxidants/chemistry ; Ethanol/chemistry ; Free Radical Scavengers/chemistry ; Free Radical Scavengers/pharmacology ; Gene Expression Regulation/drug effects ; Humans ; Inflammation/chemically induced ; Interleukin-1beta/biosynthesis ; Interleukin-6/biosynthesis ; Lipopolysaccharides/toxicity ; Mice ; Nitric Oxide/biosynthesis ; Plant Extracts/chemistry ; Plant Leaves/chemistry ; RAW 264.7 Cells ; Tumor Necrosis Factor-alpha/biosynthesis ; Water/chemistry
مستخلص: The fruit of Chaenomeles sinensis has been traditionally used in ethnomedicine for the treatment of various human ailments, including pneumonia, bronchitis, and so on, but the pharmacological applications of the leaf part of the plant have not been studied. In this study, we evaluated the various radical scavenging activities and anti-inflammatory effects of different Chaenomeles sinensis leaf (CSL) extracts. The water extract showed a higher antioxidant and radical scavenging activities. However the ethanolic extracts showed higher NO scavenging activity than water extract, therefore the ethanolic extract of CSL was examined for anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The 70% ethanol extract of CSL (CSLE) has higher anti-inflammatory activity and significantly inhibited the production of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). In addition, CSLE suppressed LPS-stimulated inducible nitric oxide synthase (iNOS) and NO production, IL-1β and phospho-STAT1 expression. In this study, we investigated the effect of CSLE on the production of inflammatory mediators through the inhibition of the TRIF-dependent pathways. Furthermore, we evaluated the role of CSLE on LPS-induced expression of pro-inflammatory cytokines, such as TNF-α, IL-1β and IL-6. Our results suggest that CSLE attenuates the LPS-stimulated inflammatory responses in macrophages through regulating the key inflammatory mechanisms, providing scientific support for its traditional uses in treating various inflammatory diseases.
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فهرسة مساهمة: Keywords: Chaenomeles sinensis leaf; RAW 264.7 cells; STAT1; TRIF-dependent pathways; anti-inflammatory
المشرفين على المادة: 0 (Anti-Inflammatory Agents)
0 (Antioxidants)
0 (Free Radical Scavengers)
0 (IL6 protein, human)
0 (Interleukin-1beta)
0 (Interleukin-6)
0 (Lipopolysaccharides)
0 (Plant Extracts)
0 (Tumor Necrosis Factor-alpha)
059QF0KO0R (Water)
31C4KY9ESH (Nitric Oxide)
3K9958V90M (Ethanol)
تواريخ الأحداث: Date Created: 20160405 Date Completed: 20161213 Latest Revision: 20200225
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC6274021
DOI: 10.3390/molecules21040422
PMID: 27043497
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules21040422