دورية أكاديمية
Differential Expression of Stem Cell Markers in Human Adamantinomatous Craniopharyngioma and Pituitary Adenoma.
| العنوان: | Differential Expression of Stem Cell Markers in Human Adamantinomatous Craniopharyngioma and Pituitary Adenoma. |
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| المؤلفون: | Chang CV; Laboratório de Hormônios e Genética Molecular - LIM/42, Divisão de Endocrinologia, FMUSP, Brasília, Brazil., Araujo RV, Cirqueira CS, Cani CM, Matushita H, Cescato VA, Fragoso MC, Bronstein MD, Zerbini MC, Mendonca BB, Carvalho LR |
| المصدر: | Neuroendocrinology [Neuroendocrinology] 2017; Vol. 104 (2), pp. 183-193. Date of Electronic Publication: 2016 Apr 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Karger Country of Publication: Switzerland NLM ID: 0035665 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0194 (Electronic) Linking ISSN: 00283835 NLM ISO Abbreviation: Neuroendocrinology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basel : Karger Original Publication: Basel. |
| مواضيع طبية MeSH: | Adenoma/*metabolism , Biomarkers, Tumor/*metabolism , Craniopharyngioma/*metabolism , Neural Stem Cells/*metabolism , Pituitary Neoplasms/*metabolism, Adenoma/pathology ; Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Craniopharyngioma/pathology ; Female ; Gene Expression ; Humans ; Hyaluronan Receptors/metabolism ; Male ; Middle Aged ; Pituitary Neoplasms/pathology ; SOX9 Transcription Factor/metabolism ; SOXB1 Transcription Factors/metabolism ; Young Adult |
| مستخلص: | Background/aims: Although craniopharyngioma (CP) is histologically benign, it is a pituitary tumour that grows rapidly and often recurs. Adamantinomatous CP (ACP) was associated with an activating mutation in β-catenin, and it has been postulated that pituitary stem cells might play a role in oncogenesis in human ACP. Stem cells have also been identified in pituitary adenoma. Our aim was to characterize the expression pattern of ABCG2, CD44, DLL4, NANOG, NOTCH2, POU5F1/OCT4, SOX2, and SOX9 stem cell markers in human ACP and pituitary adenoma. Methods and Results: We studied 33 patients (9 ACP and 24 adenoma) using real-time quantitative PCR (RT-qPCR) and immunohistochemistry. SOX9 was up-regulated in ACP, exhibiting positive immunostaining in the epithelium and stroma, with the highest expression in patients with recurrence. CD44 was overexpressed in ACP as confirmed by immunohistochemistry. SOX2 did not significantly differ among the tumour types. The RT-qPCR array showed an increased expression of MKI67,OCT4/POU5F1, and DLL4 in all tumours. NANOG was decreased in ACP. ABCG2 was down-regulated in most of the tumours. NOTCH2 was significantly decreased in the adenomas. Conclusion: Our results confirm the presence of stem cell markers in human pituitary tumours as well as the different expression patterns of ACP and adenoma. These findings suggest that ACP may originate from a more undifferentiated cell cluster. Additionally, SOX9 immunodetection in the stroma and the highest expression levels related to the relapse of patients suggest a contribution to the aggressive behaviour and high recurrence of this tumour type. (© 2016 S. Karger AG, Basel.) |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (CD44 protein, human) 0 (Hyaluronan Receptors) 0 (SOX2 protein, human) 0 (SOX9 Transcription Factor) 0 (SOX9 protein, human) 0 (SOXB1 Transcription Factors) |
| تواريخ الأحداث: | Date Created: 20160511 Date Completed: 20170509 Latest Revision: 20171116 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1159/000446072 |
| PMID: | 27161333 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1423-0194 |
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| DOI: | 10.1159/000446072 |