دورية أكاديمية
أعرض في EDS

The Modified Heparin-Binding L-Asparaginase of Wolinella succinogenes.

التفاصيل البيبلوغرافية
العنوان: The Modified Heparin-Binding L-Asparaginase of Wolinella succinogenes.
المؤلفون: Sannikova EP; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Bulushova NV; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Cheperegin SE; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Gubaydullin II; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Chestukhina GG; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Ryabichenko VV; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Zalunin IA; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Kotlova EK; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Konstantinova GE; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Kubasova TS; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Shtil AA; N. N. Blokhin Russian Cancer Research Center, Kashirskoye Shosse 24, Moscow, Russia, 115478., Pokrovsky VS; N. N. Blokhin Russian Cancer Research Center, Kashirskoye Shosse 24, Moscow, Russia, 115478., Yarotsky SV; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Efremov BD; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545., Kozlov DG; State Research Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia, 117545. dg_kozlov@genetika.ru.
المصدر: Molecular biotechnology [Mol Biotechnol] 2016 Sep; Vol. 58 (8-9), pp. 528-39.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 9423533 Publication Model: Print Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: [Cham] : Springer
Original Publication: Totowa, NJ : Humana Press, c1994-
مواضيع طبية MeSH: Antineoplastic Agents/*administration & dosage , Asparaginase/*genetics , Asparaginase/*metabolism , Heparin/*metabolism , Leukemia L5178/*drug therapy , Wolinella/*enzymology, Amino Acid Substitution ; Animals ; Antineoplastic Agents/pharmacology ; Asparaginase/administration & dosage ; Asparaginase/pharmacology ; Bacterial Proteins/administration & dosage ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Proteins/pharmacology ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Humans ; K562 Cells ; Mice ; Recombinant Proteins/metabolism ; Recombinant Proteins/pharmacology ; Wolinella/genetics ; Xenograft Model Antitumor Assays
مستخلص: The modified asparaginase Was79 was derived from the recombinant wild-type L-asparaginase of Wolinella succinogenes. The Was79 contains the amino acid substitutions V23Q and K24T responsible for the resistance to trypsinolysis and the N-terminal heparin-binding peptide KRKKKGKGLGKKR responsible for the binding to heparin and tumor K562 cells in vitro. When tested on a mouse model of Fischer lymphadenosis L5178Y, therapeutic efficacy of Was79 was significantly higher than that of reference enzymes at all single therapeutic doses used (125-8000 IU/kg). At Was79 single doses of 500-8000 IU/kg, the complete remission rate of 100 % was observed. The Was79 variant can be expressed intracellularly in E. coli as a less immunogenic formyl-methionine-free form at high per cell production levels.
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فهرسة مساهمة: Keywords: Anti-tumor activity; Heparin-binding; L-Asparaginase; Reduced glutaminase activity; Trypsinolysis resistance; Wolinella succinogenes
المشرفين على المادة: 0 (Antineoplastic Agents)
0 (Bacterial Proteins)
0 (Recombinant Proteins)
9005-49-6 (Heparin)
EC 3.5.1.1 (Asparaginase)
تواريخ الأحداث: Date Created: 20160521 Date Completed: 20170309 Latest Revision: 20181113
رمز التحديث: 20221213
DOI: 10.1007/s12033-016-9950-1
PMID: 27198565
قاعدة البيانات: MEDLINE
الوصف
تدمد:1559-0305
DOI:10.1007/s12033-016-9950-1