دورية أكاديمية
A Eukaryotic Sensor for Membrane Lipid Saturation.
| العنوان: | A Eukaryotic Sensor for Membrane Lipid Saturation. |
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| المؤلفون: | Covino R; Department of Theoretical Biophysics, Max-Planck-Institute of Biophysics, 60438 Frankfurt, Germany., Ballweg S; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany., Stordeur C; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany., Michaelis JB; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany., Puth K; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany., Wernig F; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany., Bahrami A; Department of Theoretical Biophysics, Max-Planck-Institute of Biophysics, 60438 Frankfurt, Germany., Ernst AM; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06510, USA., Hummer G; Department of Theoretical Biophysics, Max-Planck-Institute of Biophysics, 60438 Frankfurt, Germany., Ernst R; Institute of Biochemistry and Buchmann and Institute for Molecular Life Sciences, Goethe University Frankfurt, I 60438 Frankfurt, Germany. Electronic address: ernst@em.uni-frankfurt.de. |
| المصدر: | Molecular cell [Mol Cell] 2016 Jul 07; Vol. 63 (1), pp. 49-59. Date of Electronic Publication: 2016 Jun 16. |
| نوع المنشور: | Journal Article; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Ma : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1997- |
| مواضيع طبية MeSH: | Membrane Fluidity*, Endoplasmic Reticulum/*metabolism , Fatty Acids/*metabolism , Intracellular Membranes/*metabolism , Membrane Proteins/*metabolism , Saccharomyces cerevisiae/*metabolism , Saccharomyces cerevisiae Proteins/*metabolism , Transcription Factors/*metabolism, Electron Spin Resonance Spectroscopy ; Fatty Acid Desaturases/genetics ; Fatty Acid Desaturases/metabolism ; Gene Expression Regulation, Fungal ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Molecular Dynamics Simulation ; Mutation ; Protein Conformation, alpha-Helical ; Protein Stability ; Proteolysis ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Signal Transduction ; Stearoyl-CoA Desaturase ; Structure-Activity Relationship ; Transcription Factors/chemistry ; Transcription Factors/genetics ; Transcriptional Activation |
| مستخلص: | Maintaining a fluid bilayer is essential for cell signaling and survival. Lipid saturation is a key factor determining lipid packing and membrane fluidity, and it must be tightly controlled to guarantee organelle function and identity. A dedicated eukaryotic mechanism of lipid saturation sensing, however, remains elusive. Here we show that Mga2, a transcription factor conserved among fungi, acts as a lipid-packing sensor in the ER membrane to control the production of unsaturated fatty acids. Systematic mutagenesis, molecular dynamics simulations, and electron paramagnetic resonance spectroscopy identify a pivotal role of the oligomeric transmembrane helix (TMH) of Mga2 for intra-membrane sensing, and they show that the lipid environment controls the proteolytic activation of Mga2 by stabilizing alternative rotational orientations of the TMH region. This work establishes a eukaryotic strategy of lipid saturation sensing that differs significantly from the analogous bacterial mechanism relying on hydrophobic thickness. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Fatty Acids) 0 (MGA2 protein, S cerevisiae) 0 (Membrane Proteins) 0 (Saccharomyces cerevisiae Proteins) 0 (Transcription Factors) EC 1.14.19.- (Fatty Acid Desaturases) EC 1.14.19.1 (Stearoyl-CoA Desaturase) EC 1.14.99.- (delta-9 fatty acid desaturase) |
| تواريخ الأحداث: | Date Created: 20160621 Date Completed: 20170825 Latest Revision: 20201209 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.molcel.2016.05.015 |
| PMID: | 27320200 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4164 |
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| DOI: | 10.1016/j.molcel.2016.05.015 |