دورية أكاديمية
Comparative assessment of seller's staining test (SST) and direct fluorescent antibody test for rapid and accurate laboratory diagnosis of rabies.
| العنوان: | Comparative assessment of seller's staining test (SST) and direct fluorescent antibody test for rapid and accurate laboratory diagnosis of rabies. |
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| المؤلفون: | Tekki IS; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Ponfa ZN; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Nwosuh CI; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Kumbish PR; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Jonah CL; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Okewole PA; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Shamaki D; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria., Ahmed SM; National Veterinary Research Institute (NVRI), PMB 01 Vom, Nigeria. |
| المصدر: | African health sciences [Afr Health Sci] 2016 Mar; Vol. 16 (1), pp. 123-7. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Faculty of Medicine, Makerere University Country of Publication: Uganda NLM ID: 101149451 Publication Model: Print Cited Medium: Internet ISSN: 1729-0503 (Electronic) Linking ISSN: 16806905 NLM ISO Abbreviation: Afr Health Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kampala, Uganda : Faculty of Medicine, Makerere University, [2001- |
| مواضيع طبية MeSH: | Staining and Labeling*, Antibodies, Viral/*analysis , Fluorescent Antibody Technique, Direct/*methods , Rabies/*diagnosis , Rabies virus/*immunology , Rabies virus/*isolation & purification, Animals ; Dog Diseases ; Dogs ; Humans ; Nigeria ; Sensitivity and Specificity |
| مستخلص: | Background: Rabies causes 55, 000 annual human deaths globally and about 10,000 people are exposed annually in Nigeria. Diagnosis of animal rabies in most African countries has been by direct microscopic examination. In Nigeria, the Seller's stain test (SST) was employed until 2009. Before then, both SST and dFAT were used concurrently until the dFAT became the only standard method. Objective: This study was designed to assess the sensitivity and specificity of the SST in relation to the 'gold standard' dFAT in diagnosis of rabies in Nigeria. Methods: A total of 88 animal specimens submitted to the Rabies National Reference Laboratory, Nigeria were routinely tested for rabies by SST and dFAT. Results: Overall, 65.9% of the specimens were positive for rabies by SST, while 81.8% were positive by dFAT. The sensitivity of SST in relation to the gold standard dFAT was 81.0% (95% CIs; 69.7% - 88.6%), while the specificity was 100% (95% CIs; 76% - 100%). Conclusion: The relatively low sensitivity of the SST observed in this study calls for its replacement with the dFAT for accurate diagnosis of rabies and timely decisions on administration of PEP to prevent untimely deaths of exposed humans. |
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| فهرسة مساهمة: | Keywords: Nigeria; Seller's Staining Test; diagnosis; direct fluorescent antibody test; rabies |
| المشرفين على المادة: | 0 (Antibodies, Viral) |
| تواريخ الأحداث: | Date Created: 20160701 Date Completed: 20170410 Latest Revision: 20240528 |
| رمز التحديث: | 20240528 |
| مُعرف محوري في PubMed: | PMC4915429 |
| DOI: | 10.4314/ahs.v16i1.16 |
| PMID: | 27358622 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1729-0503 |
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| DOI: | 10.4314/ahs.v16i1.16 |