دورية أكاديمية
Peptide Synthesis on a Next-Generation DNA Sequencing Platform.
| العنوان: | Peptide Synthesis on a Next-Generation DNA Sequencing Platform. |
|---|---|
| المؤلفون: | Svensen N; Department of Pharmacology, Weill Cornell Medical College, Cornell University, New York, NY, 10065, USA., Peersen OB; Department of Biochemistry, Colorado State University, Fort Collins, CO, 80523, USA., Jaffrey SR; Department of Pharmacology, Weill Cornell Medical College, Cornell University, New York, NY, 10065, USA. srj2003@med.cornell.edu. |
| المصدر: | Chembiochem : a European journal of chemical biology [Chembiochem] 2016 Sep 02; Vol. 17 (17), pp. 1628-35. Date of Electronic Publication: 2016 Jul 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 100937360 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-7633 (Electronic) Linking ISSN: 14394227 NLM ISO Abbreviation: Chembiochem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim, Germany : Wiley-VCH Verlag, c2000- |
| مواضيع طبية MeSH: | High-Throughput Nucleotide Sequencing* , Peptide Biosynthesis* , Sequence Analysis, DNA*, DNA/*genetics |
| مستخلص: | Methods for displaying large numbers of peptides on solid surfaces are essential for high-throughput characterization of peptide function and binding properties. Here we describe a method for converting the >10(7) flow cell-bound clusters of identical DNA strands generated by the Illumina DNA sequencing technology into clusters of complementary RNA, and subsequently peptide clusters. We modified the flow-cell-bound primers with ribonucleotides thus enabling them to be used by poliovirus polymerase 3D(pol) . The primers hybridize to the clustered DNA thus leading to RNA clusters. The RNAs fold into functional protein- or small molecule-binding aptamers. We used the mRNA-display approach to synthesize flow-cell-tethered peptides from these RNA clusters. The peptides showed selective binding to cognate antibodies. The methods described here provide an approach for using DNA clusters to template peptide synthesis on an Illumina flow cell, thus providing new opportunities for massively parallel peptide-based assays. (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
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| معلومات مُعتمدة: | R01 AI059130 United States AI NIAID NIH HHS; R01 EB010249 United States EB NIBIB NIH HHS; R01 NS064516 United States NS NINDS NIH HHS; R37 AI059130 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: aptamers; high-throughput screening; mRNA display; next-generation DNA sequencing; peptide display; primer-dependent RNA synthesis |
| المشرفين على المادة: | 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20160708 Date Completed: 20170508 Latest Revision: 20230826 |
| رمز التحديث: | 20230826 |
| مُعرف محوري في PubMed: | PMC5183537 |
| DOI: | 10.1002/cbic.201600298 |
| PMID: | 27385640 |
| قاعدة البيانات: | MEDLINE |
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