دورية أكاديمية
Non-invasive single-cell biomechanical analysis using live-imaging datasets.
| العنوان: | Non-invasive single-cell biomechanical analysis using live-imaging datasets. |
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| المؤلفون: | Pearson YE; Department of Applied Mathematics and Sciences, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE., Lund AW; Department of Cell, Developmental and Cancer Biology, Oregon Health and Science University, Portland, OR 97239, USA., Lin AW; Endothelix, Inc., 2500 West Loop, South Houston, TX 77027, USA., Ng CP; Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore 138602 Mimetas BV, JH Oortweg 19, Leiden 2333 CH, The Netherlands., Alsuwaidi A; Department of Biomedical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE., Azzeh S; Department of Biomedical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE., Gater DL; Department of Applied Mathematics and Sciences, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE., Teo JC; Department of Biomedical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE jeremy.teo@kustar.ac.ae. |
| المصدر: | Journal of cell science [J Cell Sci] 2016 Sep 01; Vol. 129 (17), pp. 3351-64. Date of Electronic Publication: 2016 Jul 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Databases as Topic* , Imaging, Three-Dimensional*, Single-Cell Analysis/*methods, Adult ; Algorithms ; Animals ; Automation ; Biomechanical Phenomena/drug effects ; CD8-Positive T-Lymphocytes/cytology ; CD8-Positive T-Lymphocytes/drug effects ; Cell Survival/drug effects ; Elasticity ; Humans ; Ionomycin/pharmacology ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/drug effects ; Mice, Inbred C57BL ; Osteoblasts/cytology ; Osteoblasts/drug effects ; Phenotype ; Tetradecanoylphorbol Acetate/pharmacology ; Time Factors ; Viscosity |
| مستخلص: | The physiological state of a cell is governed by a multitude of processes and can be described by a combination of mechanical, spatial and temporal properties. Quantifying cell dynamics at multiple scales is essential for comprehensive studies of cellular function, and remains a challenge for traditional end-point assays. We introduce an efficient, non-invasive computational tool that takes time-lapse images as input to automatically detect, segment and analyze unlabeled live cells; the program then outputs kinematic cellular shape and migration parameters, while simultaneously measuring cellular stiffness and viscosity. We demonstrate the capabilities of the program by testing it on human mesenchymal stem cells (huMSCs) induced to differentiate towards the osteoblastic (huOB) lineage, and T-lymphocyte cells (T cells) of naïve and stimulated phenotypes. The program detected relative cellular stiffness differences in huMSCs and huOBs that were comparable to those obtained with studies that utilize atomic force microscopy; it further distinguished naïve from stimulated T cells, based on characteristics necessary to invoke an immune response. In summary, we introduce an integrated tool to decipher spatiotemporal and intracellular dynamics of cells, providing a new and alternative approach for cell characterization. (© 2016. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: Cell biomechanics; Cell migration; Live imaging; Mechanobiology; Mesenchymal stem cells; T-lymphocyte cells |
| سلسلة جزيئية: | figshare figshare.com/s/a207830096d7cdd32164 |
| المشرفين على المادة: | 56092-81-0 (Ionomycin) NI40JAQ945 (Tetradecanoylphorbol Acetate) |
| تواريخ الأحداث: | Date Created: 20160717 Date Completed: 20170808 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1242/jcs.191205 |
| PMID: | 27422102 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.191205 |