دورية أكاديمية
أعرض في EDS

Investigating the potential role of non-vls genes on linear plasmid 28-1 in virulence and persistence by Borrelia burgdorferi.

التفاصيل البيبلوغرافية
العنوان: Investigating the potential role of non-vls genes on linear plasmid 28-1 in virulence and persistence by Borrelia burgdorferi.
المؤلفون: Magunda PR; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA.; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA., Bankhead T; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA. tbankhead@vetmed.wsu.edu.; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, USA. tbankhead@vetmed.wsu.edu.
المصدر: BMC microbiology [BMC Microbiol] 2016 Aug 08; Vol. 16 (1), pp. 180. Date of Electronic Publication: 2016 Aug 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Borrelia burgdorferi/*genetics , Lyme Disease/*microbiology, Animals ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Antigens, Bacterial/metabolism ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Bacterial Proteins/genetics ; Borrelia burgdorferi/immunology ; Disease Models, Animal ; Down-Regulation ; Immune Evasion ; Lipoproteins/genetics ; Lyme Disease/immunology ; Male ; Mice ; Mice, Inbred C3H ; Mutation ; Plasmids ; Virulence
مستخلص: Background: The lp28-1 plasmid is required for persistent infection by the Lyme disease spirochete, Borrelia burgdorferi. Mutational studies on this plasmid have shown that the vls locus is important for antigenic variation of the VlsE lipoprotein that leads to immune evasion and persistence. However, it is still unknown whether the vls system is the only genetic locus on this plasmid necessary for long-term infection, and thus the potential role of non-vls genes on lp28-1 in virulence and persistence is yet to be fully determined. Despite extensive mutational analyses, two lp28-1 regions containing the ORFs bbf19 - bbf22 and bbf27 - bbf30 have not yet been mutated in their entirety.
Results: In this study, we set out to establish if these unstudied regions of lp28-1 play a role in spirochete persistence. Results show that the generated mutants were fully infectious in immunocompetent mice, and were able to persist for 91 days following infection. Following this finding, ospC expression by these mutants was determined, as it has been reported that spirochetes lacking lp28-1 fail to downregulate expression of this lipoprotein leading to immune clearance. Data presented here failed to show a definitive difference in ospC expression levels during host infection when the mutants were compared to the wild type.
Conclusions: Overall, the results strongly suggest that non-vls genes residing on lp28-1 do not play a role in spirochete persistence during infection of the mammalian host, and that the regions under study are likely not involved in the regulation of ospC expression. In conjunction with previous studies involving mutation of non-vls loci on lp28-1, these findings suggest that the vls locus is likely the sole genetic element on this plasmid responsible for immune evasion and persistence exhibited by the Lyme disease pathogen.
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فهرسة مساهمة: Keywords: Borrelia; Immune evasion; Linear plasmid 28–1; Lyme disease; Persistence
المشرفين على المادة: 0 (Antigens, Bacterial)
0 (Bacterial Outer Membrane Proteins)
0 (Bacterial Proteins)
0 (Lipoproteins)
0 (OspC protein)
0 (VlsE protein, Borrelia burgdorferi)
تواريخ الأحداث: Date Created: 20160810 Date Completed: 20170531 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4977671
DOI: 10.1186/s12866-016-0806-4
PMID: 27502325
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2180
DOI:10.1186/s12866-016-0806-4