دورية أكاديمية
أعرض في EDS

Cervicovaginal cytokines, sialidase activity and bacterial load in reproductive-aged women with intermediate vaginal flora.

التفاصيل البيبلوغرافية
العنوان: Cervicovaginal cytokines, sialidase activity and bacterial load in reproductive-aged women with intermediate vaginal flora.
المؤلفون: Santos-Greatti MMV; Department of Pathology, Botucatu Medical School, UNESP - Univ. Estadual Paulista, Botucatu, São Paulo, Brazil. Electronic address: mariana_morena@hotmail.com., da Silva MG; Department of Pathology, Botucatu Medical School, UNESP - Univ. Estadual Paulista, Botucatu, São Paulo, Brazil. Electronic address: mgsilva@fmb.unesp.br., Ferreira CST; Department of Pathology, Botucatu Medical School, UNESP - Univ. Estadual Paulista, Botucatu, São Paulo, Brazil. Electronic address: catafner@hotmail.com., Marconi C; Department of Pathology, Botucatu Medical School, UNESP - Univ. Estadual Paulista, Botucatu, São Paulo, Brazil; Department of Basic Pathology - Federal University of Paraná, Curitiba, Paraná, Brazil. Electronic address: marconi.cml@gmail.com.
المصدر: Journal of reproductive immunology [J Reprod Immunol] 2016 Nov; Vol. 118, pp. 36-41. Date of Electronic Publication: 2016 Aug 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Ireland NLM ID: 8001906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7603 (Electronic) Linking ISSN: 01650378 NLM ISO Abbreviation: J Reprod Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier/North-Holland Biomedical Press
Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Cervix Uteri/*immunology , Gardnerella vaginalis/*immunology , Gram-Positive Bacterial Infections/*immunology , Infertility, Female/*immunology , Neuraminidase/*metabolism , Vagina/*immunology , Vaginosis, Bacterial/*immunology, Adolescent ; Adult ; Aging ; Bacterial Load ; Cervix Uteri/microbiology ; Cross-Sectional Studies ; Cytokines/metabolism ; Female ; Humans ; Infertility, Female/microbiology ; Inflammation Mediators/metabolism ; Microbiota ; Middle Aged ; Pregnancy ; Principal Component Analysis ; Reproduction/immunology ; Vagina/microbiology ; Vaginosis, Bacterial/microbiology ; Young Adult
مستخلص: Studies have shown that not only bacterial vaginosis, but also intermediate vaginal flora has deleterious effects for women's reproductive health. However, literature still lacks information about microbiological and immunological aspects of intermediate flora.
Objective: To characterize intermediate flora regarding levels of Interleukin (IL)-1beta, IL-6, IL-8, tumor necrosis factor-alpha, interleukin 1 receptor antagonist (IL-1ra), IL-10, sialidase; loads of Gardnerella vaginalis, total bacteria and to verify whether it is closer related to normal flora or bacterial vaginosis. This cross-sectional study enrolled 526 non-pregnant reproductive-aged women distributed in 3 groups according to pattern of vaginal flora using Nugent's system in normal, intermediate and bacterial vaginosis. Cervicovaginal levels of cytokines, sialidases, loads of G. vaginalis and total bacteria were assessed by ELISA, conversion of MUAN and quantitative real-time PCR, respectively. A principal component analysis(PCA) using all measured parameters was performed to compare the three different types of flora. Results showed that intermediate flora is associated with increased cervicovaginal IL-1beta in relation to normal flora(P<0.0001). When compared to bacterial vaginosis, intermediate flora has higher IL-8 and IL-10 levels(P<0.01). Sialidases were in significantly lower levels in normal and intermediate flora than bacterial vaginosis(P<0.0001). Loads of G. vaginalis and total bacterial differed among all groups(P<0.0001), being highest in bacterial vaginosis. PCA showed that normal and intermediate flora were closely scattered, while bacterial vaginosis were grouped separately.
Conclusion: Although intermediate flora shows some differences in cytokines, sialidases and bacterial loads in relation to normal flora and bacterial vaginosis, when taken together, general microbiological and immunological pattern pattern of intermediate flora resembles the normal flora.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Bacterial vaginosis; Cytokine; Gardnerella vaginalis; Intermediate flora; Sialidases
المشرفين على المادة: 0 (Cytokines)
0 (Inflammation Mediators)
EC 3.2.1.18 (Neuraminidase)
تواريخ الأحداث: Date Created: 20160914 Date Completed: 20171205 Latest Revision: 20171213
رمز التحديث: 20231215
DOI: 10.1016/j.jri.2016.08.005
PMID: 27620141
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7603
DOI:10.1016/j.jri.2016.08.005