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MicroRNA-137 Inhibits EFNB2 Expression Affected by a Genetic Variant and Is Expressed Aberrantly in Peripheral Blood of Schizophrenia Patients.

التفاصيل البيبلوغرافية
العنوان: MicroRNA-137 Inhibits EFNB2 Expression Affected by a Genetic Variant and Is Expressed Aberrantly in Peripheral Blood of Schizophrenia Patients.
المؤلفون: Wu S; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China., Zhang R; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China; Translational medicine center, Honghui Hospital, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710054, China. Electronic address: zhangruity12@163.com., Nie F; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China., Wang X; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China., Jiang C; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China., Liu M; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China., Valenzuela RK; Department of Human Genetics 4, Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR), Singapore., Liu W; Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA., Shi Y; Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200030, China., Ma J; Department of Biochemistry and Molecular Biology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education, Xi'an, Shaanxi 710061, China. Electronic address: majie_article@163.com.
المصدر: EBioMedicine [EBioMedicine] 2016 Oct; Vol. 12, pp. 133-142. Date of Electronic Publication: 2016 Sep 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101647039 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2352-3964 (Electronic) Linking ISSN: 23523964 NLM ISO Abbreviation: EBioMedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2014]-
مواضيع طبية MeSH: Gene Expression Regulation* , Genetic Variation*, Ephrin-B2/*genetics , MicroRNAs/*genetics , Schizophrenia/*genetics, 3' Untranslated Regions ; Alleles ; Base Sequence ; Cell Line ; Computational Biology/methods ; Databases, Nucleic Acid ; Female ; Humans ; Male ; MicroRNAs/chemistry ; Nucleic Acid Conformation ; Polymorphism, Single Nucleotide ; RNA Interference ; RNA, Messenger/chemistry ; RNA, Messenger/genetics ; Schizophrenia/diagnosis
مستخلص: MicroRNAs (miRNAs) are a class of endogenous and non-coding single-stranded RNAs of approximately 22 nucleotides, many of which are evolutionarily conserved. Genome-wide association studies have identified a robust statistical association between the MIR137 gene and schizophrenia in Europeans, which was replicated in the Han Chinese population in a case-control study. In the previous study, we provided evidence for a significant association between the EFNB2 gene and schizophrenia in Han Chinese subjects. In the current study, we utilized computational analysis, vector construction of point mutations, luciferase reporter assays and gene expression assays including RT-qPCR and western blotting methods to investigate miR-137 directly targeting EFNB2 gene and explore the reversal effect of a genetic variant of SNP rs550067317 in the putative seed-pair region of EFNB2 3'-UTR. We also found that miR-137 could be detected in the peripheral blood of a cohort of first-onset schizophrenia patients and healthy controls, and the area under curve was 0.795 (95% confidence interval 0.700-0.890), which is a middle diagnostic value for disease, suggesting that it might be valuable for diagnosing schizophrenia. In summary, this study would improve our understanding of the role of miR-137 in schizophrenia-associated signaling pathways and identify the genetic basis of rs550067317 for schizophrenia. Furthermore, we provided new evidence for the involvement of miR-137 in the etiology and diagnosis of schizophrenia, which might contribute to the discovery of new biomarkers and therapeutic targets for the disease.
(Copyright © 2016. Published by Elsevier B.V.)
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فهرسة مساهمة: Keywords: EFNB2; MiR-137; Regulation; SNP; Schizophrenia
المشرفين على المادة: 0 (3' Untranslated Regions)
0 (EFNB2 protein, human)
0 (Ephrin-B2)
0 (MIRN137 microRNA, human)
0 (MicroRNAs)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20160922 Date Completed: 20170209 Latest Revision: 20240531
رمز التحديث: 20240531
مُعرف محوري في PubMed: PMC5078603
DOI: 10.1016/j.ebiom.2016.09.012
PMID: 27650867
قاعدة البيانات: MEDLINE
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