دورية أكاديمية
أعرض في EDS

Encapsulation into Stealth Liposomes Enhances the Antitumor Action of Recombinant Cratylia mollis Lectin Expressed in Escherichia coli .

التفاصيل البيبلوغرافية
العنوان: Encapsulation into Stealth Liposomes Enhances the Antitumor Action of Recombinant Cratylia mollis Lectin Expressed in Escherichia coli .
المؤلفون: da Cunha CR; Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil., da Silva LC; Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de PernambucoRecife, Brazil; Programa de Pós-Graduação em Biologia Parasitária, Universidade CeumaSão Luís, Brazil., Almeida FJ; Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil., Ferraz MS; Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil., Varejão N; Laboratório de Agregação de Proteínas e Amiloidoses, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil., Cartaxo MF; Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil., de Miranda RC; Programa de Pós-Graduação em Meio Ambiente, Universidade Ceuma São Luís, Brazil., de Aguiar FC Jr; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco Vitória de Santo Antão, Brazil., Santos NP; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco Vitória de Santo Antão, Brazil., Coelho LC; Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil., Santos-Magalhães NS; Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil., Correia MT; Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil.
المصدر: Frontiers in microbiology [Front Microbiol] 2016 Sep 16; Vol. 7, pp. 1355. Date of Electronic Publication: 2016 Sep 16 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: This study evaluated the in vivo antitumor potential of the recombinant lectin from seeds of Cratylia mollis (rCramoll) expressed in Escherichia coli , free or encapsulated in stealth liposomes, using mice transplanted with sarcoma 180. rCramoll-loaded stealth liposomes (rCramoll-lipo) were formulated by hydration of the lipid film followed by cycles of freezing and thawing, and about 60% of rCramoll was encapsulated. This novel preparation showed particle size, polydispersity index, and pH suitable for the evaluation of antitumor activity in vivo . Tumor growth inhibition rates were 59% for rCramoll and 75% for rCramoll-lipo. Histopathological analysis of the experimental groups showed that both free and encapsulated lectin caused no changes in the kidneys of animals. Hematological analysis revealed that treatment with rCramoll-lipo significantly increased leukocyte concentration when compared with the untreated and rCramoll group. In conclusion, the encapsulation of rCramoll in stealth liposomes improves its antitumor activity without substantial toxicity; this approach was more successful than the previous results reported for pCramoll loaded into conventional liposomes. At this point, a crucial difference between the antitumor action of free and encapsulated rCramoll was found along with their effects on immune cells. Further investigations are required to elucidate the mechanism(s) of the antitumor effect induced by rCramoll.
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فهرسة مساهمة: Keywords: cancer; heterologous expression; immunomodulation; lectins; pharmaceutical preparations
تواريخ الأحداث: Date Created: 20161004 Latest Revision: 20240325
رمز التحديث: 20240325
مُعرف محوري في PubMed: PMC5026010
DOI: 10.3389/fmicb.2016.01355
PMID: 27695439
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2016.01355