دورية أكاديمية
Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis.
| العنوان: | Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis. |
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| المؤلفون: | Hermeto LC; Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., DeRossi R; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., Oliveira RJ; Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário 'Maria Aparecida Pedrossian', Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.; Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.; Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br., Pesarini JR; Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário 'Maria Aparecida Pedrossian', Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil.; Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., Antoniolli-Silva AC; Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário 'Maria Aparecida Pedrossian', Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil.; Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., Jardim PH; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil., Santana AE; Departamento de Clínica e Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Jaboticabal, SP, Brasil., Deffune E; Laboratório de Engenharia Celular, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista 'Julio de Mesquita Filho', Botucatu, SP, Brasil., Rinaldi JC; Instituto de Biociências, Departamento de Morfologia, Laboratório de Matriz Extra-Celular, Universidade Estadual Paulista 'Julio de Mesquita Filho', Botucatu, SP, Brasil., Justulin LA; Instituto de Biociências, Departamento de Morfologia, Laboratório de Matriz Extra-Celular, Universidade Estadual Paulista 'Julio de Mesquita Filho', Botucatu, SP, Brasil. |
| المصدر: | Genetics and molecular research : GMR [Genet Mol Res] 2016 Sep 02; Vol. 15 (3). Date of Electronic Publication: 2016 Sep 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: FUNPEC Country of Publication: Brazil NLM ID: 101169387 Publication Model: Electronic Cited Medium: Internet ISSN: 1676-5680 (Electronic) Linking ISSN: 16765680 NLM ISO Abbreviation: Genet Mol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Ribeirao Preto, SP Brazil] : FUNPEC, 2002- |
| مواضيع طبية MeSH: | Chondrogenesis* , Mesenchymal Stem Cell Transplantation* , Platelet Transfusion*, Osteoarthritis/*therapy , Platelet-Rich Plasma/*cytology , Regeneration/*physiology, Adipocytes/cytology ; Adipocytes/physiology ; Animals ; Cartilage, Articular/pathology ; Cell Differentiation ; Chondrocytes/cytology ; Chondrocytes/physiology ; Collagenases ; Disease Models, Animal ; Humans ; Injections, Intra-Articular ; Knee Joint/pathology ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/physiology ; Osteoarthritis/chemically induced ; Osteoarthritis/pathology ; Osteoblasts/cytology ; Osteoblasts/physiology ; Rabbits ; Transplantation, Autologous |
| مستخلص: | The current study aims to evaluate the macroscopic and histological effects of autologous mesenchymal stem cells (MSC) and platelet-rich plasma on knee articular cartilage regeneration in an experimental model of osteoarthritis. Twenty-four rabbits were randomly divided into four groups: control group, platelet-rich plasma group, autologous MSC undifferentiated group, and autologous MSC differentiated into chondrocyte group. Collagenase solution was used to induce osteoarthritis, and treatments were applied to each group at 6 weeks following osteoarthritis induction. After 60 days of therapy, the animals were euthanized and the articular surfaces were subjected to macroscopic and histological evaluations. The adipogenic, chondrogenic, and osteogenic differentiation potentials of MSCs were evaluated. Macroscopic and histological examinations revealed improved tissue repair in the MSC-treated groups. However, no difference was found between MSC-differentiated and undifferentiated chondrocytes. We found that MSCs derived from adipose tissue and platelet-rich plasma were associated with beneficial effects in articular cartilage regeneration during experimental osteoarthritis. Competing Interests: The authors declare no conflict of interest. |
| المشرفين على المادة: | EC 3.4.24.- (Collagenases) |
| تواريخ الأحداث: | Date Created: 20161006 Date Completed: 20170223 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.4238/gmr.15038569 |
| PMID: | 27706690 |
| قاعدة البيانات: | MEDLINE |
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