دورية أكاديمية
[Platelets Decrease the Sensitivity of Leukemia Cell L1210 to Multiple Drugs via Activiting The AKT and ERK Signalling Pathway].
| العنوان: | [Platelets Decrease the Sensitivity of Leukemia Cell L1210 to Multiple Drugs via Activiting The AKT and ERK Signalling Pathway]. |
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| المؤلفون: | Hu YB; Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China., Shao LB; Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China., Zhao L; Medical College of Qingdao University, Qingdao 266071, Shandong Province, China., Shen Y; Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China., Wu K; Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China., Wang YQ; Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China. E-mail: yiqiangwang@suda.edu.cn. |
| المصدر: | Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2016 Oct; Vol. 24 (5), pp. 1489-1494. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Zhongguo shi yan xue za zhi she, |
| مواضيع طبية MeSH: | Blood Platelets*, Animals ; Cell Proliferation ; Doxorubicin ; Leukemia L1210 ; MAP Kinase Signaling System ; Methotrexate ; Mice ; Phosphorylation ; Proto-Oncogene Proteins c-akt ; Signal Transduction ; Vincristine |
| مستخلص: | Objective: To explore the effect of platelets on signal transducers and the sensitivity of leukemia cells to chemotherapeuticl drugs in leukemia cells L1210. Methods: Murine platelets were prepared and cocultured with leukemia L1210 cells, and the aggregation between them was observed by flow cytometry. The levels of several transducer proteins in leukemia cells were analyzed with Western blot. In some experiments, methotrexate, vincristine or doxorubicin was added to the coculture system and the cell proliferation was measured by using CCK8 colorigenic methods to detect the sensitivity of leukemia cells to the therapeuticals drugs. Results: Platelets, either freshly prepared or fixed with 1% paraformaldehyde, aggregated with leukemia cells. Both fresh and fixed platelets increased the level of phosphorylation of AKT and ERK in leukemia cells as measured with Western blot. Also, platelets significantly decreased the sensitivity of 3 therapeutics to L1210 cells. Conclusion: Platelets may bind with L1210 cells and decrease the sensitivity of the leukemia cells to chemotherapeutics, possibly by activating the AKT and ERK signaling pathways. |
| المشرفين على المادة: | 5J49Q6B70F (Vincristine) 80168379AG (Doxorubicin) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) YL5FZ2Y5U1 (Methotrexate) |
| تواريخ الأحداث: | Date Created: 20161028 Date Completed: 20180510 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.7534/j.issn.1009-2137.2016.05.037 |
| PMID: | 27784380 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-2137 |
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| DOI: | 10.7534/j.issn.1009-2137.2016.05.037 |