دورية أكاديمية
Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests.
| العنوان: | Metagenomic analysis of medicinal Cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests. |
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| المؤلفون: | McKernan K; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Spangler J; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Helbert Y; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Lynch RC; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Devitt-Lee A; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Zhang L; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Orphe W; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Warner J; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Foss T; Medicinal Genomics Corporation, Woburn, MA, 01801, USA., Hudalla CJ; ProVerde Laboratories, Milford, MA, 01757, USA., Silva M; ProVerde Laboratories, Milford, MA, 01757, USA., Smith DR; Medicinal Genomics Corporation, Woburn, MA, 01801, USA. |
| المصدر: | F1000Research [F1000Res] 2016 Oct 07; Vol. 5, pp. 2471. Date of Electronic Publication: 2016 Oct 07 (Print Publication: 2016). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: F1000 Research Ltd Country of Publication: England NLM ID: 101594320 Publication Model: eCollection Cited Medium: Print ISSN: 2046-1402 (Print) Linking ISSN: 20461402 NLM ISO Abbreviation: F1000Res Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: London : F1000 Research Ltd |
| مستخلص: | Background : The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods : A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA. All samples were then subjected to next-generation sequencing and metagenomics analysis to enumerate the bacteria and fungi present before and after growth on culture-based media. Results : Several pathogenic or toxigenic bacterial and fungal species were identified in proportions of >5% of classified reads on the samples, including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Ralstonia pickettii, Salmonella enterica, Stenotrophomonas maltophilia, Aspergillus ostianus, Aspergillus sydowii, Penicillium citrinum and Penicillium steckii. Samples subjected to culture showed substantial shifts in the number and diversity of species present, including the failure of Aspergillus species to grow well on either platform. Substantial growth of Clostridium botulinum and other bacteria were frequently observed on one or both of the culture-based TYM platforms. The presence of plant growth promoting (beneficial) fungal species further influenced the differential growth of species in the microbiome of each sample. Conclusions : These findings have important implications for the Cannabis and food safety testing industries. Competing Interests: KM, JS, YH, RCL, AD-L, LZ, WO, JW, TF and DRS are employees of Courtagen Life Sciences, the parent company of Medicinal Genomics, which manufactures the commercial qPCR test used in this study. |
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| فهرسة مساهمة: | Keywords: 3M-Petrifilm; Biomérieux-TEMPO; Cannabis; Illumina; PathogINDICAtor-qPCR; metagenomics; microbiome; safety |
| تواريخ الأحداث: | Date Created: 20161118 Latest Revision: 20220331 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5089129 |
| DOI: | 10.12688/f1000research.9662.1 |
| PMID: | 27853518 |
| قاعدة البيانات: | MEDLINE |
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