دورية أكاديمية
MCT8 deficiency in Purkinje cells disrupts embryonic chicken cerebellar development.
| العنوان: | MCT8 deficiency in Purkinje cells disrupts embryonic chicken cerebellar development. |
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| المؤلفون: | Delbaere J; Laboratory of Comparative EndocrinologyDepartment of Biology, KU Leuven, Leuven, Belgium., Vancamp P; Laboratory of Comparative EndocrinologyDepartment of Biology, KU Leuven, Leuven, Belgium., Van Herck SL; Laboratory of Comparative EndocrinologyDepartment of Biology, KU Leuven, Leuven, Belgium., Bourgeois NM; Laboratory of Comparative EndocrinologyDepartment of Biology, KU Leuven, Leuven, Belgium., Green MJ; Medical Research Council Centre for Developmental NeurobiologyKing's College London, London, UK., Wingate RJ; Medical Research Council Centre for Developmental NeurobiologyKing's College London, London, UK., Darras VM; Laboratory of Comparative EndocrinologyDepartment of Biology, KU Leuven, Leuven, Belgium Veerle.Darras@bio.kuleuven.be. |
| المصدر: | The Journal of endocrinology [J Endocrinol] 2017 Feb; Vol. 232 (2), pp. 259-272. Date of Electronic Publication: 2016 Nov 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioScientifica Country of Publication: England NLM ID: 0375363 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1479-6805 (Electronic) Linking ISSN: 00220795 NLM ISO Abbreviation: J Endocrinol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Bristol, UK : BioScientifica Original Publication: Bristol, UK : Society for Endocrinology |
| مواضيع طبية MeSH: | Cerebellum/*embryology , Monocarboxylic Acid Transporters/*metabolism , Neurogenesis/*genetics , Organogenesis/*genetics , Purkinje Cells/*metabolism, Animals ; Cell Movement/genetics ; Cerebellum/cytology ; Cerebellum/metabolism ; Chick Embryo ; Down-Regulation ; Embryonic Development ; Mental Retardation, X-Linked/genetics ; Mental Retardation, X-Linked/metabolism ; Monocarboxylic Acid Transporters/genetics ; Muscle Hypotonia/genetics ; Muscle Hypotonia/metabolism ; Muscular Atrophy/genetics ; Muscular Atrophy/metabolism ; Nuclear Receptor Subfamily 1, Group F, Member 1/genetics ; Nuclear Receptor Subfamily 1, Group F, Member 1/metabolism ; Purkinje Cells/cytology |
| مستخلص: | Inactivating mutations in the human SLC16A2 gene encoding the thyroid hormone transporter monocarboxylate transporter 8 (MCT8) result in the Allan-Herndon-Dudley syndrome accompanied by severe locomotor deficits. The underlying mechanisms of the associated cerebellar maldevelopment were studied using the chicken as a model. Electroporation of an MCT8-RNAi vector into the cerebellar anlage of a 3-day-old embryo allowed knockdown of MCT8 in Purkinje cell precursors. This resulted in the downregulation of the thyroid hormone-responsive gene RORα and the Purkinje cell-specific differentiation marker LHX1/5 at day 6. MCT8 knockdown also results in a smaller and less complex dendritic tree at day 18 suggesting a pivotal role of MCT8 for cell-autonomous Purkinje cell maturation. Early administration of the thyroid hormone analogue 3,5,3'-triiodothyroacetic acid partially rescued early Purkinje cell differentiation. MCT8-deficient Purkinje cells also induced non-autonomous effects as they led to a reduced granule cell precursor proliferation, a thinner external germinal layer and a loss of PAX6 expression. By contrast, at day 18, the external germinal layer thickness was increased, with an increase in presence of Axonin-1-positive post-mitotic granule cells in the initial stage of radial migration. The concomitant accumulation of presumptive migrating granule cells in the molecular layer, suggests that inward radial migration to the internal granular layer is stalled. In conclusion, early MCT8 deficiency in Purkinje cells results in both cell-autonomous and non-autonomous effects on cerebellar development and indicates that MCT8 expression is essential from very early stages of development, providing a novel insight into the ontogenesis of the Allan-Herndon-Dudley syndrome. (© 2017 Society for Endocrinology.) |
| معلومات مُعتمدة: | MR/N026063/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: AHDS; MCT8; Purkinje cells; cerebellum; chicken embryo; thyroid hormone |
| المشرفين على المادة: | 0 (Monocarboxylic Acid Transporters) 0 (Nuclear Receptor Subfamily 1, Group F, Member 1) |
| SCR Disease Name: | Allan-Herndon-Dudley syndrome |
| تواريخ الأحداث: | Date Created: 20161124 Date Completed: 20170614 Latest Revision: 20210109 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1530/JOE-16-0323 |
| PMID: | 27879339 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1479-6805 |
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| DOI: | 10.1530/JOE-16-0323 |