دورية أكاديمية
أعرض في EDS

Potential contribution of the uterine microbiome in the development of endometrial cancer.

التفاصيل البيبلوغرافية
العنوان: Potential contribution of the uterine microbiome in the development of endometrial cancer.
المؤلفون: Walther-António MR; Department of Surgery, Mayo Clinic, Rochester, MN, 55905, USA., Chen J; Department of Health Sciences Research, Mayo Clinic, Rochester, MN, 55905, USA., Multinu F; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, 55905, USA., Hokenstad A; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, 55905, USA., Distad TJ; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA., Cheek EH; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA., Keeney GL; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA., Creedon DJ; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, 55905, USA.; Present Address: North Memorial Medical Center, Robbinsdale, MN, 55442, USA., Nelson H; Department of Surgery, Mayo Clinic, Rochester, MN, 55905, USA.; Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA., Mariani A; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, 55905, USA. Mariani.Andrea@mayo.edu., Chia N; Department of Surgery, Mayo Clinic, Rochester, MN, 55905, USA. Chia.Nicholas@mayo.edu.; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA. Chia.Nicholas@mayo.edu.; Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA. Chia.Nicholas@mayo.edu.
المصدر: Genome medicine [Genome Med] 2016 Nov 25; Vol. 8 (1), pp. 122. Date of Electronic Publication: 2016 Nov 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101475844 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-994X (Electronic) Linking ISSN: 1756994X NLM ISO Abbreviation: Genome Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central
مواضيع طبية MeSH: Bacteria/*classification , Endometrial Hyperplasia/*microbiology , Endometrial Neoplasms/*microbiology , Sequence Analysis, DNA/*methods , Uterus/*microbiology, Adult ; Aged ; Bacteria/genetics ; Bacteria/isolation & purification ; DNA, Bacterial/analysis ; DNA, Ribosomal/analysis ; Fallopian Tubes/microbiology ; Female ; Humans ; Middle Aged ; Ovary/microbiology ; Phylogeny ; RNA, Ribosomal, 16S/analysis ; Risk Factors ; Urine/microbiology ; Vagina/microbiology
مستخلص: Background: Endometrial cancer studies have led to a number of well-defined but mechanistically unconnected genetic and environmental risk factors. One of the emerging modulators between environmental triggers and genetic expression is the microbiome. We set out to inquire about the composition of the uterine microbiome and its putative role in endometrial cancer.
Methods: We undertook a study of the microbiome in samples taken from different locations along the female reproductive tract in patients with endometrial cancer (n = 17), patients with endometrial hyperplasia (endometrial cancer precursor, n = 4), and patients afflicted with benign uterine conditions (n = 10). Vaginal, cervical, Fallopian, ovarian, peritoneal, and urine samples were collected aseptically both in the operating room and the pathology laboratory. DNA extraction was followed by amplification and high-throughput next generation sequencing (MiSeq) of the 16S rDNA V3-V5 region to identify the microbiota present. Microbiota data were summarized using both α-diversity to reflect species richness and evenness within bacterial populations and β-diversity to reflect the shared diversity between bacterial populations. Statistical significance was determined through the use of multiple testing, including the generalized mixed-effects model.
Results: The microbiome sequencing (16S rDNA V3-V5 region) revealed that the microbiomes of all organs (vagina, cervix, Fallopian tubes, and ovaries) are significantly correlated (p < 0.001) and that there is a structural microbiome shift in the cancer and hyperplasia cases, distinguishable from the benign cases (p = 0.01). Several taxa were found to be significantly enriched in samples belonging to the endometrial cancer cohort: Firmicutes (Anaerostipes, ph2, Dialister, Peptoniphilus, 1-68, Ruminococcus, and Anaerotruncus), Spirochaetes (Treponema), Actinobacteria (Atopobium), Bacteroidetes (Bacteroides and Porphyromonas), and Proteobacteria (Arthrospira). Of particular relevance, the simultaneous presence of Atopobium vaginae and an uncultured representative of the Porphyromonas sp. (99 % match to P. somerae) were found to be associated with disease status, especially if combined with a high vaginal pH (>4.5).
Conclusions: Our results suggest that the detection of A. vaginae and the identified Porphyromonas sp. in the gynecologic tract combined with a high vaginal pH is statistically associated with the presence of endometrial cancer. Given the documented association of the identified microorganisms with other pathologies, these findings raise the possibility of a microbiome role in the manifestation, etiology, or progression of endometrial cancer that should be further investigated.
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معلومات مُعتمدة: P50 CA136393 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: 16S rDNA; Atopobium; Endometrial cancer; Microbiome; Porphyromonas; Uterus
المشرفين على المادة: 0 (DNA, Bacterial)
0 (DNA, Ribosomal)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20161126 Date Completed: 20170309 Latest Revision: 20190817
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5123330
DOI: 10.1186/s13073-016-0368-y
PMID: 27884207
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-994X
DOI:10.1186/s13073-016-0368-y