دورية أكاديمية
Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems.
| العنوان: | Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems. |
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| المؤلفون: | Dimura M; Chair for Molecular Physical Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany; Institute for Pharmaceutical and Medicinal Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany., Peulen TO; Chair for Molecular Physical Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany., Hanke CA; Chair for Molecular Physical Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany., Prakash A; Chair for Molecular Physical Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany., Gohlke H; Institute for Pharmaceutical and Medicinal Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany., Seidel CA; Chair for Molecular Physical Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany. Electronic address: cseidel@hhu.de. |
| المصدر: | Current opinion in structural biology [Curr Opin Struct Biol] 2016 Oct; Vol. 40, pp. 163-185. Date of Electronic Publication: 2016 Dec 07. |
| نوع المنشور: | Journal Article; Review; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9107784 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-033X (Electronic) Linking ISSN: 0959440X NLM ISO Abbreviation: Curr Opin Struct Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: London : Elsevier Science Original Publication: London, UK : Current Biology, 1991- |
| مواضيع طبية MeSH: | Models, Molecular*, Biochemistry/*methods , Fluorescence Resonance Energy Transfer/*methods, Animals ; Humans |
| مستخلص: | Förster Resonance Energy Transfer (FRET) combined with single-molecule spectroscopy probes macromolecular structure and dynamics and identifies coexisting conformational states. We review recent methodological developments in integrative structural modeling by satisfying spatial restraints on networks of FRET pairs (hybrid-FRET). We discuss procedures to incorporate prior structural knowledge and to obtain optimal distance networks. Finally, a workflow for hybrid-FRET is presented that automates integrative structural modeling and experiment planning to put hybrid-FRET on rails. To test this workflow, we simulate realistic single-molecule experiments and resolve three protein conformers, exchanging at 30μs and 10ms, with accuracies of 1-3Å RMSD versus the target structure. Incorporation of data from other spectroscopies and imaging is also discussed. (Copyright © 2016 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| تواريخ الأحداث: | Date Created: 20161213 Date Completed: 20171206 Latest Revision: 20180223 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.sbi.2016.11.012 |
| PMID: | 27939973 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-033X |
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| DOI: | 10.1016/j.sbi.2016.11.012 |