دورية أكاديمية
Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR.
| العنوان: | Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR. |
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| المؤلفون: | Hanron AE; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA., Billman ZP; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA; Center for Emerging and Re-emerging Infectious Diseases, University of Washington, Seattle, WA, USA., Seilie AM; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA., Chang M; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA., Murphy SC; Department of Laboratory Medicine, University of Washington, Seattle, WA, USA; Center for Emerging and Re-emerging Infectious Diseases, University of Washington, Seattle, WA, USA; Department of Microbiology, University of Washington, Seattle, WA, USA. Electronic address: murphysc@uw.edu. |
| المصدر: | Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Mar; Vol. 87 (3), pp. 226-228. Date of Electronic Publication: 2016 Dec 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [New York, NY] : Elsevier Biomedical, [c1983- |
| مواضيع طبية MeSH: | Babesiosis/*diagnosis , DNA, Protozoan/*genetics , DNA, Ribosomal/*genetics , RNA, Ribosomal, 18S/*genetics , Reverse Transcriptase Polymerase Chain Reaction/*methods, Babesia microti/isolation & purification ; Cross Infection/blood ; Cross Infection/parasitology ; Humans ; Serologic Tests/methods ; Transfusion Reaction |
| مستخلص: | Babesia are increasingly appreciated as a cause of transfusion-transmitted infection. Sensitive methods are needed to screen blood products. We report herein that B. microti 18S rRNA is over 1,000-fold more abundant than its coding genes, making reverse transcription PCR (RT-PCR) much more sensitive than PCR. Babesia 18S rRNA may be useful for screening the blood supply. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 18S rRNA; Babesia; Clinical microbiology; Diagnostics; RT-PCR; Transfusion |
| المشرفين على المادة: | 0 (DNA, Protozoan) 0 (DNA, Ribosomal) 0 (RNA, Ribosomal, 18S) |
| تواريخ الأحداث: | Date Created: 20161218 Date Completed: 20170217 Latest Revision: 20171116 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.diagmicrobio.2016.11.021 |
| PMID: | 27986353 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1879-0070 |
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| DOI: | 10.1016/j.diagmicrobio.2016.11.021 |