دورية أكاديمية
أعرض في EDS

VEGF gene therapy cooperatively recruits molecules from the immune system and stimulates cell homing and angiogenesis in refractory angina.

التفاصيل البيبلوغرافية
العنوان: VEGF gene therapy cooperatively recruits molecules from the immune system and stimulates cell homing and angiogenesis in refractory angina.
المؤلفون: Eibel B; Laboratório de Cardiologia Molecular e Celular (Serviço de Medicina Experimental)/Secretaria de Cirurgia, Instituto de Cardiologia/Fundação Universitária de Cardiologia (IC/FUC), Brazil., Markoski MM; Laboratório de Cardiologia Molecular e Celular (Serviço de Medicina Experimental)/Secretaria de Cirurgia, Instituto de Cardiologia/Fundação Universitária de Cardiologia (IC/FUC), Brazil., Rodrigues CG; Laboratório de Cardiologia Molecular e Celular (Serviço de Medicina Experimental)/Secretaria de Cirurgia, Instituto de Cardiologia/Fundação Universitária de Cardiologia (IC/FUC), Brazil., Dipp T; Faculdade de Desenvolvimento do Rio Grande do Sul (FADERGS), Brazil., de Salles FB; Instituto do Coração/Faculdade de Medicina da Universidade de São Paulo (INCOR/FMUSP), Brazil., Giusti II; Laboratório de Cardiologia Molecular e Celular (Serviço de Medicina Experimental)/Secretaria de Cirurgia, Instituto de Cardiologia/Fundação Universitária de Cardiologia (IC/FUC), Brazil., Nardi NB; Universidade Luterana do Brasil (ULBRA), Brazil., Plentz RD; Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil., Kalil RA; Laboratório de Cardiologia Molecular e Celular (Serviço de Medicina Experimental)/Secretaria de Cirurgia, Instituto de Cardiologia/Fundação Universitária de Cardiologia (IC/FUC), Brazil; Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil. Electronic address: kalil.renato@gmail.com.
المصدر: Cytokine [Cytokine] 2017 Mar; Vol. 91, pp. 44-50. Date of Electronic Publication: 2016 Dec 18.
نوع المنشور: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0023 (Electronic) Linking ISSN: 10434666 NLM ISO Abbreviation: Cytokine Subsets: MEDLINE
أسماء مطبوعة: Publication: <2001- > : Oxford : Elsevier Science Ltd.
Original Publication: [Philadelphia, PA] : Saunders Scientific Publications, W.B. Saunders, [c1989-
مواضيع طبية MeSH: Angina Pectoris*/genetics , Angina Pectoris*/immunology , Angina Pectoris*/therapy , Coronary Artery Disease*/genetics , Coronary Artery Disease*/immunology , Coronary Artery Disease*/therapy , Genetic Therapy* , Neovascularization, Physiologic*/genetics , Neovascularization, Physiologic*/immunology , Paracrine Communication*/genetics , Paracrine Communication*/immunology , Vascular Endothelial Growth Factor A*/genetics , Vascular Endothelial Growth Factor A*/immunology, Endothelial Progenitor Cells/*immunology, Aged ; Female ; Humans ; Male ; Middle Aged
مستخلص: Background: New vessels are formed in response to stimuli from angiogenic factors, a process in which paracrine signaling is fundamental.
Objective: To investigate the cooperative paracrine signaling profile in response to Vascular Endothelial Growth Factor (VEGF) gene therapy in patients with coronary artery disease (CAD) and refractory angina.
Method: A cohort study was conducted in which plasma was collected from patients who underwent gene therapy with a plasmid expressing VEGF 165 (10) and from surgical procedure controls (4). Blood samples were collected from both groups prior to baseline and on days 3, 9 and 27 after the interventions and subjected to systemic analysis of protein expression (Interleukin-6, IL-6; Tumor Necrosis Factor-α, TNF-α; Interleukin-10, IL-10; Stromal Derived Factor-1 α, SDF-1α; VEGF; Angiopoietin-1, ANGPT-1; and Endothelin-1, ET-1) using the enzyme-linked immunosorbent assay (ELISA).
Results: Analysis showed an increase in proinflammatory IL-6 (p=0.02) and ET-1 (p=0.05) on day 3 after gene therapy and in VEGF (p=0.02) on day 9. A strong positive correlation was found between mobilization of endothelial progenitor cells and TNF-α on day 9 (r=0.71; p=0.03). Furthermore, a strong correlation between β-blockers, antiplatelets, and vasodilators with SDF-1α baseline in the group undergoing gene therapy was verified (r=0.74; p=0.004).
Conclusion: Analysis of cooperative paracrine signaling after VEGF gene therapy suggests that the immune system cell and angiogenic molecule expression as well as the endothelial progenitor cell mobilization are time-dependent, influenced by chronic inflammatory process and continuous pharmacological treatment.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Angiogenesis; Cell homing; Coronary artery disease; Gene therapy; Refractory angina; Vascular endothelial growth factor
المشرفين على المادة: 0 (VEGFA protein, human)
0 (Vascular Endothelial Growth Factor A)
تواريخ الأحداث: Date Created: 20161221 Date Completed: 20180105 Latest Revision: 20180210
رمز التحديث: 20240829
DOI: 10.1016/j.cyto.2016.12.005
PMID: 27997860
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0023
DOI:10.1016/j.cyto.2016.12.005