دورية أكاديمية
Factors affecting the mechanical behavior of collagen hydrogels for skin tissue engineering.
| العنوان: | Factors affecting the mechanical behavior of collagen hydrogels for skin tissue engineering. |
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| المؤلفون: | Pensalfini M; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zurich, Leonhardstrasse 21, 8092 Zurich, Switzerland., Ehret AE; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zurich, Leonhardstrasse 21, 8092 Zurich, Switzerland; Empa, Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600 Dübendorf, Switzerland., Stüdeli S; Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, August-Forel-Strasse 7, 8008 Zurich, Switzerland., Marino D; Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, August-Forel-Strasse 7, 8008 Zurich, Switzerland., Kaech A; Center for Microscopy and Image Analysis, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland., Reichmann E; Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, August-Forel-Strasse 7, 8008 Zurich, Switzerland., Mazza E; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zurich, Leonhardstrasse 21, 8092 Zurich, Switzerland; Empa, Swiss Federal Laboratories for Materials Science and Technology, Überlandstrasse 129, 8600 Dübendorf, Switzerland. |
| المصدر: | Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2017 May; Vol. 69, pp. 85-97. Date of Electronic Publication: 2016 Dec 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101322406 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0180 (Electronic) Linking ISSN: 18780180 NLM ISO Abbreviation: J Mech Behav Biomed Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Skin, Artificial* , Tissue Engineering*, Collagen/*analysis , Hydrogels/*analysis, Biocompatible Materials/analysis ; Biomechanical Phenomena ; Fibroblasts ; Humans ; Skin ; Stress, Mechanical |
| مستخلص: | The effect of the production factors yielding a functional dermal substitute was investigated by means of monotonic and cyclic uniaxial tensile tests, as well as electron microscopy visualizations. The role of (i) plastic compression, (ii) product incubation, and (iii) cell permanence in the collagenous matrix in order to achieve a skin-like behavior were characterized in terms of material and structural stiffness, in-plane kinematics, and cyclic response, as well as pore size and network density. The plastic compression resulted in a denser and stiffer material, while no corresponding change was observed in the behavior of the entire structure. This was related to the progressive reduction in product thickness and amount of excess water, rather than to formation of new crosslinks between fibers. Contrary, irrespective of the presence of human fibroblasts, the product incubation induced both material and structural stiffening, indicating the formation of a denser network. These results were confirmed by similar evolutions in the construct in-plane kinematics and cyclic stress reduction. Finally, comparison of constructs incubated in different culture media indicated a determinant contribution of the biochemical environment, rather than of the seeded cells, to the achieved mechanical properties. The observed features are relevant in terms of mechanical biocompatibility of the implant and might direct future optimizations of the production process in order to rapidly attain the desired mechanical properties. (Copyright © 2016 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Collagen hydrogel; Multiscale biomechanics; Skin substitute; Tissue engineering; Uniaxial testing |
| المشرفين على المادة: | 0 (Biocompatible Materials) 0 (Hydrogels) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20170102 Date Completed: 20180116 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jmbbm.2016.12.004 |
| PMID: | 28040611 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-0180 |
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| DOI: | 10.1016/j.jmbbm.2016.12.004 |