دورية أكاديمية
Photodynamic Inactivation of Cariogenic Pathogens Using Curcumin as Photosensitizer.
| العنوان: | Photodynamic Inactivation of Cariogenic Pathogens Using Curcumin as Photosensitizer. |
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| المؤلفون: | Araújo NC; 1 Department of Restorative Dentistry, Dental School of Pernambuco, University of Pernambuco (UPE) , Camaragibe, Brazil ., de Menezes RF; 2 Department of Endodontics, University of Pernambuco (UPE) , Camaragibe, Brazil .; 3 Center for Biophotonics, University of California , Davis (UCDavis), Sacramento, California., Carneiro VSM; 1 Department of Restorative Dentistry, Dental School of Pernambuco, University of Pernambuco (UPE) , Camaragibe, Brazil .; 7 Faculty of Dentistry, University Center Tabosa de Almeida ASCES-UNITA, Caruaru, Brazil ., Dos Santos-Neto AP; 2 Department of Endodontics, University of Pernambuco (UPE) , Camaragibe, Brazil ., Fontana CR; 4 Department of Clinical Analysis, School of Pharmaceutical Sciences , Universidade Estadual Paulista (UNESP), Araraquara, Brazil ., Bagnato VS; 5 Optics Group from Physics Institute of São Carlos (IFSC), University of São Paulo (USP) , São Carlos, Brazil ., Harvey CM; 3 Center for Biophotonics, University of California , Davis (UCDavis), Sacramento, California., Gerbi MEM; 6 Laser Center, University of Pernambuco (UPE) , Camaragibe, Brazil . |
| المصدر: | Photomedicine and laser surgery [Photomed Laser Surg] 2017 May; Vol. 35 (5), pp. 259-263. Date of Electronic Publication: 2017 Jan 12. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101222340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8550 (Electronic) Linking ISSN: 15495418 NLM ISO Abbreviation: Photomed Laser Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-c2018 |
| مواضيع طبية MeSH: | Curcumin/*pharmacology , Dental Caries/*drug therapy , Photochemotherapy/*methods , Photosensitizing Agents/*pharmacology, Analysis of Variance ; Dental Caries/microbiology ; Humans ; In Vitro Techniques ; Lactobacillus acidophilus/drug effects ; Lactobacillus acidophilus/pathogenicity ; Microbial Sensitivity Tests ; Molar/drug effects ; Molar/microbiology ; Streptococcus mutans/drug effects ; Streptococcus mutans/pathogenicity |
| مستخلص: | Objective: This investigation assessed the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to Photodynamic Therapy (PDT) when grown simultaneously in dentine carious lesions. Background Data: PDT is a technique that utilizes light to activate photosensitizers in the presence of oxygen to produce reactive radicals. Materials and Methods: A culture medium of 1% glucose, 2% sucrose, 1% young primary culture of L. acidophilus 10 8 CFU/mL, and S. mutans 10 8 CFU/mL was utilized to inoculate the bacterial induced caries on human dentine slabs. Different concentrations of the photosensitizer (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were activated through exposure to the light-emitting diode source with a central wavelength of 450 nm and a fluency of 5.7 J/cm 2 . Two light intensities (19 and 47.5 mW/cm 2 ) were tested. Four different groups were analyzed: L-D- (control group), L-D+ (drug group), L+D+1 (PDT group 1, light intensity of 19 mW/cm 2 ), and L+D+2 (PDT group 2, light intensity of 47.5 mW/cm 2 ). ANOVA/Tukey tests were utilized to compare groups (α = 5%). Results: Both light intensities required 5.0 g/L of curcumin for significant bacterial reduction (p < 0.05). No significant effect was found for L-D+, thus proving the absence of a potential inherent toxicity. Conclusions: Curcumin has a toxic effect on microorganisms at appreciable concentrations upon photoactivation. However, it was required to use the maximum concentration of the drug for a successful procedure. |
| فهرسة مساهمة: | Keywords: dentin; dentistry; photodynamic therapy |
| المشرفين على المادة: | 0 (Photosensitizing Agents) IT942ZTH98 (Curcumin) |
| تواريخ الأحداث: | Date Created: 20170117 Date Completed: 20180910 Latest Revision: 20180910 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1089/pho.2016.4156 |
| PMID: | 28092483 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-8550 |
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| DOI: | 10.1089/pho.2016.4156 |