دورية أكاديمية
Intestinal transport and absorption of bioactive phenolic compounds from a chemically characterized aqueous extract of Athrixia phylicoides.
| العنوان: | Intestinal transport and absorption of bioactive phenolic compounds from a chemically characterized aqueous extract of Athrixia phylicoides. |
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| المؤلفون: | Bowles SL; Biomedical Research and Innovation Platform, South African Medical Research Council, P.O. Box 19070, Tygerberg 7505, South Africa. Electronic address: Sandra.Bowles@mrc.ac.za., Ntamo Y; Biomedical Research and Innovation Platform, South African Medical Research Council, P.O. Box 19070, Tygerberg 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa. Electronic address: Yonela.Ntamo@mrc.ac.za., Malherbe CJ; Plant Bioactives Group, Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa. Electronic address: MalherbeCh@arc.agric.za., Kappo AM; Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa. Electronic address: KappoA@unizulu.ac.za., Louw J; Biomedical Research and Innovation Platform, South African Medical Research Council, P.O. Box 19070, Tygerberg 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa. Electronic address: Johan.Louw@mrc.ac.za., Muller CJ; Biomedical Research and Innovation Platform, South African Medical Research Council, P.O. Box 19070, Tygerberg 7505, South Africa; Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa; Department of Medical Physiology, Stellenbosch University, Tygerberg 7507, South Africa. Electronic address: Christo.Muller@mrc.ac.za. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2017 Mar 22; Vol. 200, pp. 45-50. Date of Electronic Publication: 2017 Feb 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Asteraceae*, Hydroxybenzoates/*chemistry , Intestinal Absorption/*drug effects , Phenols/*chemistry , Plant Extracts/*chemistry, Caco-2 Cells ; Dose-Response Relationship, Drug ; Humans ; Hydroxybenzoates/isolation & purification ; Hydroxybenzoates/pharmacology ; Intestinal Absorption/physiology ; Phenols/isolation & purification ; Phenols/pharmacology ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Plant Leaves ; Plant Stems |
| مستخلص: | Ethnopharmacological Relevance: Athrixia phylicoides, popularly known as "bush tea", is an indigenous aromatic shrub found in mountainous and grassland areas of the northern and eastern parts of southern Africa. The plant is traditionally used for the treatment of several ailments, including coughing, treating infected wounds, treating boils and sore throat, hypertension and heart disease. Potential anti-diabetic effects have also been demonstrated in vitro. Aim of the Study: To investigate the intestinal transport of prominent phenolic constituents, across a fully differentiated Caco-2 monolayer, using a characterized aqueous extract of A. phylicoides, previously shown to have bioactivity. Materials and Methods: HPLC-DAD and LC/MS analyses were used to identify the major phenolic compounds within the extract. Intestinal transport of the phenolic compounds was assessed using a differentiated Caco-2 monolayer model in order to predict bioavailability and identify metabolite formation. Rate of transport, efflux and percentage cross-over were calculated for the respective phenolic compounds. Results: Nine prominent compounds, present in the aqueous extract of A. phylicoides, were identified. Of these, three phenolic acids (protocatechuic acid, caffeic acid and para-coumaric acid), crossed the Caco-2 cell monolayer in significant amounts, with P Conclusions: Para-Coumaric acid, identified for the first time in A. phylicoides, was shown to have the highest predicted bioavailability suggesting that it could play a major role in the bioactivity of A. phylicoides. (Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 3,4 di-O-caffeoylquinic acid (PubChem CID: 5281780); 3,5 di-O-caffeoylquinic acid (PubChem CID: 6474310); 4,5 di-O-caffeoylquinic acid (PubChem CID: 6474309); Athrixia phylicoides; Caco-2 transport; Caffeic acid; Para-coumaric acid; Protocatechuic acid; caffeic acid (PubChem CID: 689043); chlorogenic acid (PubChem CID: 1794427); cryptochlorogenic acid (PubChem CID: 9798666); neochlorogenic acid (PubChem CID: 5280633); para-coumaric acid (PubChem CID: 637542); protocatechuic acid (PubChem CID: 72) |
| المشرفين على المادة: | 0 (Hydroxybenzoates) 0 (Phenols) 0 (Plant Extracts) I3P9R8317T (phenolic acid) |
| تواريخ الأحداث: | Date Created: 20170219 Date Completed: 20170719 Latest Revision: 20180816 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jep.2017.02.019 |
| PMID: | 28213108 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2017.02.019 |