دورية أكاديمية
Wnt proteins synergize to activate β-catenin signaling.
| العنوان: | Wnt proteins synergize to activate β-catenin signaling. |
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| المؤلفون: | Alok A; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore., Lei Z; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore.; Centre for Computational Biology, Duke-NUS Medical School, 169857 Singapore., Jagannathan NS; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore.; Centre for Computational Biology, Duke-NUS Medical School, 169857 Singapore., Kaur S; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore., Harmston N; Centre for Computational Biology, Duke-NUS Medical School, 169857 Singapore., Rozen SG; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore.; Centre for Computational Biology, Duke-NUS Medical School, 169857 Singapore., Tucker-Kellogg L; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore.; Centre for Computational Biology, Duke-NUS Medical School, 169857 Singapore., Virshup DM; Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, 169857 Singapore david.virshup@duke-nus.edu.sg.; Department of Pediatrics, Duke University School of Medicine, Durham, NC, USA. |
| المصدر: | Journal of cell science [J Cell Sci] 2017 May 01; Vol. 130 (9), pp. 1532-1544. Date of Electronic Publication: 2017 Mar 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Signal Transduction*/genetics, Wnt Proteins/*metabolism , beta Catenin/*metabolism, Acetylation ; Clone Cells ; Gene Expression Regulation, Neoplastic ; HEK293 Cells ; HeLa Cells ; Humans ; Low Density Lipoprotein Receptor-Related Protein-6/metabolism ; Models, Biological ; Phosphorylation ; Protein Stability ; Receptors, G-Protein-Coupled/metabolism ; Stomach Neoplasms/genetics ; Up-Regulation/genetics |
| مستخلص: | Wnt ligands are involved in diverse signaling pathways that are active during development, maintenance of tissue homeostasis and in various disease states. While signaling regulated by individual Wnts has been extensively studied, Wnts are rarely expressed alone, and the consequences of Wnt gene co-expression are not well understood. Here, we studied the effect of co-expression of Wnts on the β-catenin signaling pathway. While some Wnts are deemed 'non-canonical' due to their limited ability to activate β-catenin when expressed alone, unexpectedly, we find that multiple Wnt combinations can synergistically activate β-catenin signaling in multiple cell types. WNT1- and WNT7B-mediated synergistic Wnt signaling requires FZD5, FZD8 and LRP6, as well as the WNT7B co-receptors GPR124 (also known as ADGRA2) and RECK. Unexpectedly, this synergistic signaling occurs downstream of β-catenin stabilization, and is correlated with increased lysine acetylation of β-catenin. Wnt synergy provides a general mechanism to confer increased combinatorial control over this important regulatory pathway. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2017. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: Cancer; Development; GPCR; Synergy; Wnt signaling |
| المشرفين على المادة: | 0 (ADGRA2 protein, human) 0 (LRP6 protein, human) 0 (Low Density Lipoprotein Receptor-Related Protein-6) 0 (Receptors, G-Protein-Coupled) 0 (Wnt Proteins) 0 (beta Catenin) |
| تواريخ الأحداث: | Date Created: 20170315 Date Completed: 20180122 Latest Revision: 20191210 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1242/jcs.198093 |
| PMID: | 28289266 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.198093 |