دورية أكاديمية
أعرض في EDS

PX-18 Protects Human Saphenous Vein Endothelial Cells under Arterial Blood Pressure.

التفاصيل البيبلوغرافية
العنوان: PX-18 Protects Human Saphenous Vein Endothelial Cells under Arterial Blood Pressure.
المؤلفون: Kupreishvili K; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands., Stooker W; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands; Department of Cardiac Surgery, OLVG, Amsterdam, The Netherlands., Emmens RW; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands., Vonk ABA; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands; Department of Cardiac Surgery, VU University Medical Center, Amsterdam, The Netherlands., Sipkens JA; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands., van Dijk A; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands., Eijsman L; Department of Cardiac Surgery, VU University Medical Center, Amsterdam, The Netherlands., Quax PH; Einthoven Laboratory of Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands., van Hinsbergh VWM; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands; Department of Physiology, VU University Medical Center, Amsterdam, The Netherlands., Krijnen PAJ; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands. Electronic address: paj.krijnen@vumc.nl., Niessen HWM; Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands; Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, Amsterdam, The Netherlands; Department of Cardiac Surgery, VU University Medical Center, Amsterdam, The Netherlands.
المصدر: Annals of vascular surgery [Ann Vasc Surg] 2017 Jul; Vol. 42, pp. 293-298. Date of Electronic Publication: 2017 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8703941 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-5947 (Electronic) Linking ISSN: 08905096 NLM ISO Abbreviation: Ann Vasc Surg Subsets: MEDLINE
أسماء مطبوعة: Publication: <2007->: Netherlands : Elsevier
Original Publication: Detroit : [Published by Expansion scientifique française for Annals of Vascular Surgery, Inc. and Association pour la promotion de la chirurgie vasculaire, Paris, c1986-
مواضيع طبية MeSH: Arterial Pressure*, Alkanesulfonic Acids/*pharmacology , Endothelial Cells/*drug effects , Group II Phospholipases A2/*antagonists & inhibitors , Mechanotransduction, Cellular/*drug effects , Oleic Acids/*pharmacology , Phospholipase A2 Inhibitors/*pharmacology , Saphenous Vein/*drug effects, Apoptosis/drug effects ; Cells, Cultured ; Endothelial Cells/enzymology ; Endothelial Cells/pathology ; Group II Phospholipases A2/metabolism ; Human Umbilical Vein Endothelial Cells/drug effects ; Human Umbilical Vein Endothelial Cells/enzymology ; Human Umbilical Vein Endothelial Cells/pathology ; Humans ; Saphenous Vein/enzymology ; Saphenous Vein/pathology ; Time Factors
مستخلص: Background: Arterial blood pressure-induced shear stress causes endothelial cell apoptosis and inflammation in vein grafts after coronary artery bypass grafting. As the inflammatory protein type IIA secretory phospholipase A 2 (sPLA 2 -IIA) has been shown to progress atherosclerosis, we hypothesized a role for sPLA 2 -IIA herein.
Methods: The effects of PX-18, an inhibitor of both sPLA 2 -IIA and apoptosis, on residual endothelium and the presence of sPLA 2 -IIA were studied in human saphenous vein segments (n = 6) perfused at arterial blood pressure with autologous blood for 6 hrs.
Results: The presence of PX-18 in the perfusion blood induced a significant 20% reduction in endothelial cell loss compared to veins perfused without PX18, coinciding with significantly reduced sPLA 2 -IIA levels in the media of the vein graft wall. In addition, PX-18 significantly attenuated caspase-3 activation in human umbilical vein endothelial cells subjected to shear stress via mechanical stretch independent of sPLA 2 -IIA.
Conclusions: In conclusion, PX-18 protects saphenous vein endothelial cells from arterial blood pressure-induced death, possibly also independent of sPLA 2 -IIA inhibition.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
المشرفين على المادة: 0 (2-(N,N-bis(oleoyloxyethyl)amino)-1-ethanesulfonic acid)
0 (Alkanesulfonic Acids)
0 (Oleic Acids)
0 (Phospholipase A2 Inhibitors)
EC 3.1.1.4 (Group II Phospholipases A2)
EC 3.1.1.4 (PLA2G2A protein, human)
تواريخ الأحداث: Date Created: 20170317 Date Completed: 20180402 Latest Revision: 20180402
رمز التحديث: 20231215
DOI: 10.1016/j.avsg.2016.10.063
PMID: 28300679
قاعدة البيانات: MEDLINE
الوصف
تدمد:1615-5947
DOI:10.1016/j.avsg.2016.10.063