دورية أكاديمية
أعرض في EDS

Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation.

التفاصيل البيبلوغرافية
العنوان: Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation.
المؤلفون: Mol EA; Department of Cardiology, Laboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands., Goumans MJ; Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, the Netherlands., Doevendans PA; Department of Cardiology, Laboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands; UMC Utrecht Regenerative Medicine Center, University Medical Center, Utrecht, the Netherlands; Netherlands Heart Institute (ICIN), Utrecht, the Netherlands., Sluijter JPG; Department of Cardiology, Laboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands; UMC Utrecht Regenerative Medicine Center, University Medical Center, Utrecht, the Netherlands; Netherlands Heart Institute (ICIN), Utrecht, the Netherlands., Vader P; Laboratory of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, the Netherlands. Electronic address: pvader@umcutrecht.nl.
المصدر: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2017 Aug; Vol. 13 (6), pp. 2061-2065. Date of Electronic Publication: 2017 Mar 30.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Elsevier, c2005-
مواضيع طبية MeSH: Chromatography, Gel/*methods , Extracellular Vesicles/*metabolism , Myocytes, Cardiac/*metabolism , Stem Cells/*metabolism , Ultracentrifugation/*methods, Cells, Cultured ; Humans ; Myocytes, Cardiac/cytology ; Stem Cells/cytology
مستخلص: Extracellular vesicles (EVs) are nano-sized, lipid bilayer-enclosed particles involved in intercellular communication. EVs are increasingly being considered as drug delivery vehicles or as cell-free approach to regenerative medicine. However, one of the major challenges for their clinical application is finding a scalable EV isolation method that yields functional EVs. Although the golden standard for EV isolation is ultracentrifugation (UC), a recent study suggested that isolation using size-exclusion chromatography (SEC) yielded EVs with more intact biophysical properties. Whether this also leads to differences in functionality remained to be investigated. Therefore, we investigated possible differences in functionality of cardiomyocyte progenitor cell-derived EVs isolated using UC and SEC. Western blot analysis showed higher pERK/ERK ratios in endothelial cells after stimulation with SEC-EVs compared to UC-EVs, indicating that SEC-EVs bear higher functionality. Therefore, we propose to use SEC-EVs for further investigation of EVs' therapeutic potential. Further optimization of isolation protocols may accelerate clinical adoption of therapeutic EVs.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Exosomes; Extracellular vesicles; Functionality; Size-exclusion chromatography; Ultracentrifugation
تواريخ الأحداث: Date Created: 20170403 Date Completed: 20180606 Latest Revision: 20180606
رمز التحديث: 20221213
DOI: 10.1016/j.nano.2017.03.011
PMID: 28365418
قاعدة البيانات: MEDLINE
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