دورية أكاديمية
أعرض في EDS

Role of NADPH Oxidase-4 in Human Endothelial Progenitor Cells.

التفاصيل البيبلوغرافية
العنوان: Role of NADPH Oxidase-4 in Human Endothelial Progenitor Cells.
المؤلفون: Hakami NY; Centre for Eye Research Australia, Royal Victorian Eye and Ear HospitalEast Melbourne, VIC, Australia; Ophthalmology, University of Melbourne, Department of SurgeryEast Melbourne, VIC, Australia; Department of Pharmacology and Therapeutics, University of MelbourneMelbourne, VIC, Australia; Faculty of Applied Medical Sciences, King Abdulaziz UniversityJeddah, Saudi Arabia., Ranjan AK; Cardiology, Icahn School of Medicine at Mount Sinai Hospital New York, NY, USA., Hardikar AA; Diabetes and Islet Biology, NHMRC Clinical Trials Centre, University of Sydney Sydney, NSW, Australia., Dusting GJ; Centre for Eye Research Australia, Royal Victorian Eye and Ear HospitalEast Melbourne, VIC, Australia; Ophthalmology, University of Melbourne, Department of SurgeryEast Melbourne, VIC, Australia., Peshavariya HM; Centre for Eye Research Australia, Royal Victorian Eye and Ear HospitalEast Melbourne, VIC, Australia; Ophthalmology, University of Melbourne, Department of SurgeryEast Melbourne, VIC, Australia.
المصدر: Frontiers in physiology [Front Physiol] 2017 Mar 23; Vol. 8, pp. 150. Date of Electronic Publication: 2017 Mar 23 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101549006 Publication Model: eCollection Cited Medium: Print ISSN: 1664-042X (Print) Linking ISSN: 1664042X NLM ISO Abbreviation: Front Physiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Introduction: Endothelial progenitor cells (EPCs) display a unique ability to promote angiogenesis and restore endothelial function in injured blood vessels. NADPH oxidase 4 (NOX4)-derived hydrogen peroxide (H 2 O 2 ) serves as a signaling molecule and promotes endothelial cell proliferation and migration as well as protecting against cell death. However, the role of NOX4 in EPC function is not completely understood. Methods: EPCs were isolated from human saphenous vein and mammary artery discarded during bypass surgery. NOX4 gene and protein expression in EPCs were measured by real time-PCR and Western blot analysis respectively. NOX4 gene expression was inhibited using an adenoviral vector expressing human NOX4 shRNA (Ad-NOX4i). H 2 O 2 production was measured by Amplex red assay. EPC migration was evaluated using a transwell migration assay. EPC proliferation and viability were measured using trypan blue counts. Results: Inhibition of NOX4 using Ad-NOX4i reduced Nox4 gene and protein expression as well as H 2 O 2 formation in EPCs. Inhibition of NOX4-derived H 2 O 2 decreased both proliferation and migration of EPCs. Interestingly, pro-inflammatory cytokine tumor necrosis factor alpha (TNFα) decreased NOX4 expression and reduced survival of EPCs. However, the survival of EPCs was further diminished by TNF-α in NOX4-knockdown cells, suggesting that NOX4 has a protective role in EPCs. Conclusion: These findings suggest that NOX4-type NADPH oxidase is important for proliferation and migration functions of EPCs and protects against pro-inflammatory cytokine induced EPC death. These properties of NOX4 may facilitate the efficient function of EPCs which is vital for successful neovascularization.
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فهرسة مساهمة: Keywords: H2O2; NOX4; ROS; TNFα; endothelial progenitor cells
تواريخ الأحداث: Date Created: 20170408 Latest Revision: 20200930
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5362645
DOI: 10.3389/fphys.2017.00150
PMID: 28386230
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-042X
DOI:10.3389/fphys.2017.00150