دورية أكاديمية
Stability of different influenza subtypes: How can high hydrostatic pressure be a useful tool for vaccine development?
العنوان: | Stability of different influenza subtypes: How can high hydrostatic pressure be a useful tool for vaccine development? |
---|---|
المؤلفون: | Dumard CH; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Barroso SPC; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Santos ACV; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Alves NS; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Couceiro JNSS; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil., Gomes AMO; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Santos PS; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil., Silva JL; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil. Electronic address: jerson@bioqmed.ufrj.br., Oliveira AC; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil; Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Brazil. Electronic address: cheble@bioqmed.ufrj.br. |
المصدر: | Biophysical chemistry [Biophys Chem] 2017 Dec; Vol. 231, pp. 116-124. Date of Electronic Publication: 2017 Apr 06. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Science B.V Country of Publication: Netherlands NLM ID: 0403171 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4200 (Electronic) Linking ISSN: 03014622 NLM ISO Abbreviation: Biophys Chem Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science B.V Original Publication: Amsterdam, North-Holland Pub. Co. |
مواضيع طبية MeSH: | Influenza A virus/*chemistry, Animals ; Guanidine/chemistry ; Humans ; Hydrostatic Pressure ; Influenza A Virus, H1N1 Subtype/chemistry ; Influenza A Virus, H1N1 Subtype/physiology ; Influenza A Virus, H3N2 Subtype/chemistry ; Influenza A Virus, H3N2 Subtype/physiology ; Influenza A Virus, H3N8 Subtype/chemistry ; Influenza A Virus, H3N8 Subtype/physiology ; Influenza A virus/physiology ; Temperature ; Urea/chemistry ; Vaccines/immunology ; Virus Inactivation |
مستخلص: | Background: Avian influenza A viruses can cross naturally into mammals and cause severe diseases, as observed for H5N1. The high lethality of human infections causes major concerns about the real risk of a possible pandemic of severe diseases to which human susceptibility may be high and universal. High hydrostatic pressure (HHP) is a valuable tool for studies regarding the folding of proteins and the assembly of macromolecular structures such as viruses; furthermore, HHP has already been demonstrated to promote viral inactivation. Methods: Here, we investigated the structural stability of avian and human influenza viruses using spectroscopic and light-scattering techniques. We found that both particles have similar structural stabilities and that HHP promotes structural changes. Results: HHP induced slight structural changes to both human and avian influenza viruses, and these changes were largely reversible when the pressure returned to its initial level. The spectroscopic data showed that H3N2 was more pressure-sensitive than H3N8. Structural changes did not predict changes in protein function, as H3N2 fusion activity was not affected, while H3N8 fusion activity drastically decreased. The fusion activity of H1N1 was also strongly affected by HHP. In all cases, HHP caused inactivation of the different influenza viruses. Conclusions: HHP may be a useful tool for vaccine development, as it induces minor and reversible structural changes that may be associated with partial preservation of viral biological activities and may potentiate their immunogenic response while abolishing their infectivity. We also confirmed that, although pressure does not promote drastic changes in viral particle structure, it can distinctly affect viral fusion activity. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
فهرسة مساهمة: | Keywords: High hydrostatic pressure; Human and avian influenza; Influenza stability; Influenza vaccine; Pressure inactivation of viruses |
المشرفين على المادة: | 0 (Vaccines) 8W8T17847W (Urea) JU58VJ6Y3B (Guanidine) |
تواريخ الأحداث: | Date Created: 20170416 Date Completed: 20171229 Latest Revision: 20171229 |
رمز التحديث: | 20221213 |
DOI: | 10.1016/j.bpc.2017.04.002 |
PMID: | 28410940 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!