دورية أكاديمية
Uric acid increases cellular and humoral alloimmunity in primary human peripheral blood mononuclear cells.
| العنوان: | Uric acid increases cellular and humoral alloimmunity in primary human peripheral blood mononuclear cells. |
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| المؤلفون: | Eleftheriadis T; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece., Pissas G; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece., Sounidaki M; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece., Antoniadi G; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece., Antoniadis N; Organ Transplant Unit, Hippokration General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece., Liakopoulos V; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece., Stefanidis I; Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa, Greece. |
| المصدر: | Nephrology (Carlton, Vic.) [Nephrology (Carlton)] 2018 Jul; Vol. 23 (7), pp. 610-615. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Science Country of Publication: Australia NLM ID: 9615568 Publication Model: Print Cited Medium: Internet ISSN: 1440-1797 (Electronic) Linking ISSN: 13205358 NLM ISO Abbreviation: Nephrology (Carlton) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Carlton, Vic., Australia : Blackwell Science, |
| مواضيع طبية MeSH: | Autoimmunity/*drug effects , Immunity, Cellular/*drug effects , Immunity, Humoral/*drug effects , Leukocytes, Mononuclear/*drug effects , Lymphocyte Activation/*drug effects , Uric Acid/*pharmacology, Adult ; Cell Proliferation/drug effects ; Cells, Cultured ; Humans ; Isoantibodies/immunology ; Isoantibodies/metabolism ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Lymphocyte Culture Test, Mixed |
| مستخلص: | Aim: Hyperuricaemia is common among kidney transplant recipients and has been associated with worse graft outcome. Since episodes of acute cellular rejection and chronic humoral rejection contribute to decreased graft survival, in this study the effect of uric acid on cellular and humoral alloimmunity was evaluated. Methods: Cellular alloimmunity was assessed by cell proliferation in two-way mixed lymphocyte reaction (MLR) with human peripheral blood mononuclear cells (PBMC). For assessing humoral alloimmunity we developed a method in which humoral alloimmunity was induced in one-way MLR. Then the de novo production of alloantibodies was measured with an antibody-mediated complement-dependent cytotoxicity assay, in which supernatants from the above MRLs were used against resting PBMC similar to the stimulator cells of the above MLRs. Results: Uric acid at a concentration above its crystallization threshold increased cellular proliferation in two-way MLRs. Supernatants from one-way MLRs performed in the presence of uric acid were more cytotoxic against PBMC from individuals that had conferred the stimulator cells for the above MLRs. Conclusions: Uric acid increases both cellular and humoral alloimmunity in human PBMC. These results offer a possible pathogenetic mechanism for the observed relation between hyperuricaemia and worse kidney allograft survival. (© 2017 Asian Pacific Society of Nephrology.) |
| فهرسة مساهمة: | Keywords: Uric acid; cellular alloimmunity; humoral alloimmunity; transplantation |
| المشرفين على المادة: | 0 (Isoantibodies) 268B43MJ25 (Uric Acid) |
| تواريخ الأحداث: | Date Created: 20170507 Date Completed: 20181126 Latest Revision: 20181126 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/nep.13069 |
| PMID: | 28477373 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1440-1797 |
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| DOI: | 10.1111/nep.13069 |