دورية أكاديمية
A broad-host-range vector system for cloning and translational lacZ fusion analysis.
| العنوان: | A broad-host-range vector system for cloning and translational lacZ fusion analysis. |
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| المؤلفون: | Tai TN; University of Illinois, Department of Microbiology, Urbana 61801., Havelka WA, Kaplan S |
| المصدر: | Plasmid [Plasmid] 1988 May; Vol. 19 (3), pp. 175-88. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 7802221 Publication Model: Print Cited Medium: Print ISSN: 0147-619X (Print) Linking ISSN: 0147619X NLM ISO Abbreviation: Plasmid Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Cloning, Molecular* , Genes* , Genes, Bacterial* , Genetic Vectors* , Plasmids* , Protein Biosynthesis*, Galactosidases/*genetics , Rhodobacter sphaeroides/*genetics , beta-Galactosidase/*genetics, Base Sequence ; DNA Restriction Enzymes ; Drug Resistance, Microbial/genetics ; Escherichia coli/genetics ; Genotype ; Molecular Sequence Data ; Phenotype |
| مستخلص: | A broad-host-range vector system for studying translational fusions was constructed. The region that retains the origin of replication, nic, mob, and rep genes of the broad-host-range plasmid RSF1010 was isolated as either an HincII or a PstI-PvuII restriction fragment. These restriction fragments were ligated to tetracycline, kanamycin, or streptomycin/spectinomycin resistance genes to generate plasmids pUI501, pUI511, pUI504, and pUI506. A functional lacZ gene lacking downstream lac operon sequences together with the lac promoter was constructed from plasmids pMC1871 and pUC18. This lacZ gene was inserted into pUI501 and pUI511 to generate plasmids pUI502, pUI503, pUI512, and pUI513. An oligodeoxynucleotide sequence that carries three unique blunt-end restriction sites was synthesized, annealed, and ligated in frame to the amino-terminal end of the lacZ gene in each of these plasmids. This multiple cloning sequence will allow translational fusions to the lacZ gene in all three reading frames. The stability of these plasmids and the expression of the lacZ gene in both Escherichia coli and Rhodobacter sphaeroides were studied. |
| المشرفين على المادة: | EC 3.1.21.- (DNA Restriction Enzymes) EC 3.2.1.- (Galactosidases) EC 3.2.1.23 (beta-Galactosidase) |
| تواريخ الأحداث: | Date Created: 19880501 Date Completed: 19890413 Latest Revision: 20191022 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/0147-619x(88)90037-6 |
| PMID: | 2852814 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0147-619X |
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| DOI: | 10.1016/0147-619x(88)90037-6 |