دورية أكاديمية
Immunohistochemical, morphological and histometrical analyses of follicular development in Astyanax bimaculatus (Teleostei: Characidae) exposed to an organochlorine insecticide.
| العنوان: | Immunohistochemical, morphological and histometrical analyses of follicular development in Astyanax bimaculatus (Teleostei: Characidae) exposed to an organochlorine insecticide. |
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| المؤلفون: | Marcon L; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, 36570-000 Viçosa, MG, Brasil; Programa de Pós-graduação em Biologia de Vertebrados, PUC-Minas, Av. Dom José Gaspar, 500, Coração Eucarístico, 30535-610 Belo Horizonte, MG, Brasil. Electronic address: kalukasss@hotmail.com., Thomé RG; Departamento de Morfologia, Universidade Federal de São João Del-Rei, Campus Centro-Oeste. Rua Sebastião Gonçalves Coelho, 400, Chanadour, 35501-296 Divinópolis, MG, Brasil., Mounteer AH; Departamento de Engenharia Civil, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, 36570-000 Viçosa, MG, Brasil., Bazzoli N; Programa de Pós-graduação em Biologia de Vertebrados, PUC-Minas, Av. Dom José Gaspar, 500, Coração Eucarístico, 30535-610 Belo Horizonte, MG, Brasil., Rizzo E; Departamento de Morfologia, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Campus UFMG, 31270-901 Belo Horizonte, MG, Brasil., Benjamin LDA; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, 36570-000 Viçosa, MG, Brasil. Electronic address: laercio@ufv.br. |
| المصدر: | Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2017 Sep; Vol. 143, pp. 249-258. Date of Electronic Publication: 2017 May 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7805381 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2414 (Electronic) Linking ISSN: 01476513 NLM ISO Abbreviation: Ecotoxicol Environ Saf Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Netherlands : Elsevier |
| مواضيع طبية MeSH: | Characidae/*growth & development , Endosulfan/*toxicity , Environmental Monitoring/*methods , Insecticides/*toxicity , Ovarian Follicle/*drug effects , Water Pollutants, Chemical/*toxicity, Animals ; Dose-Response Relationship, Drug ; Female ; Immunohistochemistry ; Ovarian Follicle/metabolism ; Ovarian Follicle/pathology ; Reproduction/drug effects |
| مستخلص: | Thiodan ® is an organochlorine insecticide used in agriculture that can reach aquatic ecosystems where it can affect fish reproduction. This research aimed to evaluate follicular development and the expression of integrin β1, collagen type IV and caspase 3 by morphological, histometrical and immunohistochemical analyses of Astyanax bimaculatus exposed to Thiodan ® . Treatments included three sublethal concentrations of Thiodan ® (1.15, 2.30, and 5.60μgL -1 ) for 96h and a control without the insecticide. Mature females with ovaries in advanced follicular development were chosen for study (average weight: 11.52 ± 2.0g; average total length: 9.12 ± 0.64cm). The follicles of A. bimaculatus exhibited normal morphology, while the diameters of secondary follicles showed an increase (P< 0.05) in all concentrations when compared to the control group; a characteristic of follicles undergoing the initial stages of intoxication. Immunohistochemical analysis revealed secondary follicles with greater expression of integrin β1 and collagen type IV in cytoplasm of follicular cells than in the primary follicles in all treatments and in the control. Immunolocalization of caspase 3 was detected in follicular cells during secondary development in all tested concentrations of Thiodan ® and the control. These analyses demonstrate positive immunolocalization throughout the course of follicular development, even in fish exposed to varying concentrations of Thiodan ® for 96h, demonstrating that follicular cells retain their physiological integrity. (Copyright © 2017 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aquatic ecotoxicology; Extracellular matrix; Fish reproduction; Ovarian follicle |
| المشرفين على المادة: | 0 (Insecticides) 0 (Water Pollutants, Chemical) OKA6A6ZD4K (Endosulfan) |
| تواريخ الأحداث: | Date Created: 20170529 Date Completed: 20170928 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ecoenv.2017.05.029 |
| PMID: | 28551582 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2414 |
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| DOI: | 10.1016/j.ecoenv.2017.05.029 |