دورية أكاديمية
Technical feasibility of isolated Bowman layer graft preparation by femtosecond laser: a pilot study.
| العنوان: | Technical feasibility of isolated Bowman layer graft preparation by femtosecond laser: a pilot study. |
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| المؤلفون: | Parker JS; Netherlands Institute for Innovative Ocular Surgery, Rotterdam - The Netherlands.; Melles Cornea Clinic Rotterdam, Rotterdam - The Netherlands.; Parker Cornea, Birmingham, Alabama - USA., Huls F; University of Michigan, Ann Arbor, Michigan - USA., Cooper E; Parker Cornea, Birmingham, Alabama - USA., Graves P; Alabama Eye Bank, Birmingham, Alabama - USA., Groeneveld-van Beek EA; Netherlands Institute for Innovative Ocular Surgery, Rotterdam - The Netherlands.; Amnitrans Eye Bank Rotterdam, Rotterdam - The Netherlands., Lie J; Netherlands Institute for Innovative Ocular Surgery, Rotterdam - The Netherlands.; Amnitrans Eye Bank Rotterdam, Rotterdam - The Netherlands., Melles GRJ; Netherlands Institute for Innovative Ocular Surgery, Rotterdam - The Netherlands.; Melles Cornea Clinic Rotterdam, Rotterdam - The Netherlands.; Amnitrans Eye Bank Rotterdam, Rotterdam - The Netherlands. |
| المصدر: | European journal of ophthalmology [Eur J Ophthalmol] 2017 Nov 08; Vol. 27 (6), pp. 675-677. Date of Electronic Publication: 2017 May 26. |
| نوع المنشور: | Journal Article; Randomized Controlled Trial |
| اللغة: | English |
| بيانات الدورية: | Publisher: SAGE Publishing Country of Publication: United States NLM ID: 9110772 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1724-6016 (Electronic) Linking ISSN: 11206721 NLM ISO Abbreviation: Eur J Ophthalmol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2018- : Thousand Oaks, CA : SAGE Publishing Original Publication: Milano ; Birmingham, AL : Wichtig, c1991- |
| مواضيع طبية MeSH: | Tissue Donors*, Bowman Membrane/*surgery , Corneal Transplantation/*methods , Keratoconus/*surgery , Laser Therapy/*methods , Tissue and Organ Harvesting/*methods, Aged ; Bowman Membrane/ultrastructure ; Feasibility Studies ; Female ; Humans ; Male ; Microscopy, Electron, Transmission ; Pilot Projects ; Reproducibility of Results |
| مستخلص: | Purpose: To evaluate the technical feasibility of isolated Bowman layer (BL) graft preparation by femtosecond laser (FSL) and to compare the ultrastructural morphology to manually dissected grafts. Methods: Five whole globes were placed in custom-made eye holders and debrided of epithelium. After programming a dissection depth of 20 μm, the FSL was docked into position and 5 isolated BL grafts were created. From 5 additional globes, corneoscleral buttons were procured, mounted in artificial anterior chambers, and stripped of BL via the previously described manual technique. Three specimens from both series were randomly selected and assigned to transmission electron microscopy for ultrastructural evaluation and thickness measurements. Results: All dissections were uneventful and 10 total grafts were produced: 5 by FSL and 5 by manual dissection. Mean graft thickness was 37 (±8.6) μm (n = 3) for the FSL group and 11.7 (±1.6) μm (n = 3) for the manually dissected group. Transmission electron microscopy revealed a thick but relatively smooth posterior cut edge in the FSL group, versus a virtually isolated BL with irregular rests of dispersed stroma in the manually dissected group. Conclusions: Femtosecond laser may have potential for harvesting intact BL and with a smooth posterior surface, but accompanied by variable amounts of anterior stroma owing to technical limitations. |
| تواريخ الأحداث: | Date Created: 20170603 Date Completed: 20180810 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.5301/ejo.5000990 |
| PMID: | 28574133 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1724-6016 |
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| DOI: | 10.5301/ejo.5000990 |