دورية أكاديمية
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PRIMA: a gene-centered, RNA-to-protein method for mapping RNA-protein interactions.

التفاصيل البيبلوغرافية
العنوان: PRIMA: a gene-centered, RNA-to-protein method for mapping RNA-protein interactions.
المؤلفون: Tamburino AM; Program in Systems Biology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA., Kaymak E; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA., Shrestha S; Program in Systems Biology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA., Holdorf AD; Program in Systems Biology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA., Ryder SP; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA., Walhout AJM; Program in Systems Biology and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
المصدر: Translation (Austin, Tex.) [Translation (Austin)] 2017 Feb 28; Vol. 5 (1), pp. e1295130. Date of Electronic Publication: 2017 Feb 28 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101601190 Publication Model: eCollection Cited Medium: Print ISSN: 2169-074X (Print) Linking ISSN: 21690731 NLM ISO Abbreviation: Translation (Austin) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin, Texas : Landes Bioscience, [2013]-
مستخلص: Interactions between RNA binding proteins (RBPs) and mRNAs are critical to post-transcriptional gene regulation. Eukaryotic genomes encode thousands of mRNAs and hundreds of RBPs. However, in contrast to interactions between transcription factors (TFs) and DNA, the interactome between RBPs and RNA has been explored for only a small number of proteins and RNAs. This is largely because the focus has been on using 'protein-centered' (RBP-to-RNA) interaction mapping methods that identify the RNAs with which an individual RBP interacts. While powerful, these methods cannot as of yet be applied to the entire RBPome. Moreover, it may be desirable for a researcher to identify the repertoire of RBPs that can interact with an mRNA of interest-in a 'gene-centered' manner-yet few such techniques are available. Here, we present Protein-RNA Interaction Mapping Assay (PRIMA) with which an RNA 'bait' can be tested versus multiple RBP 'preys' in a single experiment. PRIMA is a translation-based assay that examines interactions in the yeast cytoplasm, the cellular location of mRNA translation. We show that PRIMA can be used with small RNA elements, as well as with full-length Caenorhabditis elegans 3' UTRs. PRIMA faithfully recapitulated numerous well-characterized RNA-RBP interactions and also identified novel interactions, some of which were confirmed in vivo . We envision that PRIMA will provide a complementary tool to expand the depth and scale with which the RNA-RBP interactome can be explored.
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معلومات مُعتمدة: R01 GM117237 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: 3′ untranslated region; RNA; RNA binding protein; caenorhabditis elegans; yeast
تواريخ الأحداث: Date Created: 20170714 Latest Revision: 20201001
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5501385
DOI: 10.1080/21690731.2017.1295130
PMID: 28702278
قاعدة البيانات: MEDLINE
الوصف
تدمد:2169-074X
DOI:10.1080/21690731.2017.1295130