دورية أكاديمية
أعرض في EDS

CART modulates beta-amyloid metabolism-associated enzymes and attenuates memory deficits in APP/PS1 mice.

التفاصيل البيبلوغرافية
العنوان: CART modulates beta-amyloid metabolism-associated enzymes and attenuates memory deficits in APP/PS1 mice.
المؤلفون: Yin K; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Jin J; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Zhu X; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Yu L; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Wang S; b Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine , Nanjing , China., Qian L; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Han L; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China., Xu Y; a Department of Neurology , Affiliated Drum Tower Hospital of Nanjing University Medical School , Nanjing , China.; b Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine , Nanjing , China.
المصدر: Neurological research [Neurol Res] 2017 Oct; Vol. 39 (10), pp. 885-894. Date of Electronic Publication: 2017 Jul 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 7905298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1743-1328 (Electronic) Linking ISSN: 01616412 NLM ISO Abbreviation: Neurol Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2016- : Abingdon : Taylor & Francis
Original Publication: New York, Crane, Russak.
مواضيع طبية MeSH: Alzheimer Disease/*drug therapy , Hippocampus/*drug effects , Memory Disorders/*drug therapy , Nerve Tissue Proteins/*pharmacology , Neuroprotective Agents/*pharmacology , Nootropic Agents/*pharmacology, Alzheimer Disease/metabolism ; Amyloid beta-Peptides/metabolism ; Amyloid beta-Protein Precursor/genetics ; Amyloid beta-Protein Precursor/metabolism ; Animals ; Cells, Cultured ; Disease Models, Animal ; Hippocampus/metabolism ; Humans ; Male ; Maze Learning/drug effects ; Maze Learning/physiology ; Memory Disorders/metabolism ; Mice, Transgenic ; Peptide Fragments/metabolism ; Presenilin-1/genetics ; Presenilin-1/metabolism ; Random Allocation ; Spatial Memory/drug effects ; Spatial Memory/physiology
مستخلص: Introduction: Cocaine- and amphetamine-regulated transcript (CART) peptide has been demonstrated to exert neuroprotective effects in stroke and some neurodegeneration diseases. In current study, we investigated the protective effects and underlying mechanisms of CART in APP/PS1 mice.
Methods: The protein levels of CART, soluble Aβ 1-40 and Aβ 1-42 were measured in the hippocampus of APP/PS1 mice by enzyme-linked immunosorbent assay. We determined the mRNA and protein levels of Aβ metabolism-associated enzymes including neprilysin (NEP), insulin-degrading enzyme (IDE), receptor for advanced glycation end products (RAGE), and low-density lipoprotein receptor-related protein 1 (LRP-1) in the hippocampus of APP/PS1 mice using real-time PCR and western blotting. Spatial memory was measured in APP/PS1 mice using the Morris water maze. The phosphorylation of AKT, ERK, p38, and JNK was determined using western blotting.
Results: The levels of soluble Aβ 1-40 and Aβ 1-42 were significantly decreased in the hippocampus of APP/PS1 mice after CART treatment. CART modulated the levels of NEP, IDE, RAGE, and LRP-1. In addition, CART inhibited the MAPK pathways and activated the AKT pathway, whereas inhibition of the AKT pathway decreased the levels of IDE and LRP-1. Furthermore, CART attenuated spatial memory deficits in the APP/PS1 mice.
Conclusion: CART decreases the levels of soluble Aβ in the hippocampus of APP/PS1 mice by modulating the expression of Aβ metabolism-associated enzymes, which may be associated with the MAPK and AKT pathways.
فهرسة مساهمة: Keywords: AD: Alzheimer’s disease; AKT pathway; APP: amyloid precursor protein; Alzheimer’s disease; Aβ: β-amyloid; CART; CART: Cocaine- and amphetamine-regulated transcript; ECE-1: endothelin-converting enzyme-1; ERK: extracellular signal-regulated kinases; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IDE: insulin-degrading enzyme; JNK: c-Jun N-terminal kinases; LRP-1: low-density lipoprotein receptor-related protein 1; MAPK pathways; MAPK: mitogen-activated protein kinase; NEP: neprilysin; RAGE: receptor for advanced glycation end products; beta-amyloid
المشرفين على المادة: 0 (APP protein, human)
0 (Amyloid beta-Peptides)
0 (Amyloid beta-Protein Precursor)
0 (Nerve Tissue Proteins)
0 (Neuroprotective Agents)
0 (Nootropic Agents)
0 (PSEN1 protein, human)
0 (Peptide Fragments)
0 (Presenilin-1)
0 (amyloid beta-protein (1-40))
0 (amyloid beta-protein (1-42))
0 (cocaine- and amphetamine-regulated transcript protein)
تواريخ الأحداث: Date Created: 20170727 Date Completed: 20180515 Latest Revision: 20180515
رمز التحديث: 20240829
DOI: 10.1080/01616412.2017.1348689
PMID: 28743230
قاعدة البيانات: MEDLINE
الوصف
تدمد:1743-1328
DOI:10.1080/01616412.2017.1348689