دورية أكاديمية
Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing.
| العنوان: | Role of plasma-derived fibrin on keratinocyte and fibroblast wound healing. |
|---|---|
| المؤلفون: | Law JX; Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, 56000, Kuala Lumpur, Malaysia., Chowdhury SR; Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, 56000, Kuala Lumpur, Malaysia., Aminuddin BS; Ear, Nose and Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Ampang, Selangor, Malaysia., Ruszymah BHI; Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000, Kuala Lumpur, Malaysia. ruszyidrus@gmail.com. |
| المصدر: | Cell and tissue banking [Cell Tissue Bank] 2017 Dec; Vol. 18 (4), pp. 585-595. Date of Electronic Publication: 2017 Jul 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 100965121 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6814 (Electronic) Linking ISSN: 13899333 NLM ISO Abbreviation: Cell Tissue Bank Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht, the Netherlands : Kluwer Academic Publishers, c2000- |
| مواضيع طبية MeSH: | Fibrin/*metabolism , Fibroblasts/*metabolism , Keratinocytes/*cytology , Wound Healing/*physiology, Cell Movement/physiology ; Cell Proliferation/physiology ; Cells, Cultured ; Collagen Type I/metabolism ; Extracellular Matrix/metabolism ; Humans |
| مستخلص: | Fibrin has excellent biocompatibility and biological properties to support tissue regeneration and promote wound healing. However, the role of diluted fibrin in wound healing has yet to be elucidated as it is commonly used in high concentration. This study was aimed to examine the effects of diluted plasma-derived fibrin (PDF) on keratinocyte and fibroblast wound healing in term of cell proliferation, migration, extracellular matrix (ECM) production and soluble factor secretion. Two PDF concentrations, 10 and 20% (v/v) were tested on keratinocytes and fibroblasts indirectly co-cultured in the transwell system. The control group was cultured with 5% FBS. Results showed that PDF reduced the keratinocyte growth rate and fibroblast migration, and increased the fibroblast ECM gene expression whereby significant differences were found between the 20% PDF group and the 5% FBS group. Similar trend was seen for the 10% PDF group but the differences were not significant. Comparison of the soluble factors between the PDF groups demonstrated that the level of growth-related oncogene alpha, interleukin-8 and epithelial neutrophil-activating peptide-78 were significantly higher in the 10% PDF group, whilst interleukin-1 alpha and granulocyte-macrophage colony stimulating factor were significantly more concentrated in the 20% PDF group. Our results suggested that PDF selectively elevated the expression of collagen type 1 and collagen type 3 in fibroblasts but slowed down the migration in concentration-dependent manner. These novel findings provide new insight into the role of PDF in wound healing and may have important implications for the use of fibrin in skin tissue engineering. |
| فهرسة مساهمة: | Keywords: Cell migration; Collagen; Fibrin; Fibroblast; Keratinocyte; Wound healing |
| المشرفين على المادة: | 0 (Collagen Type I) 9001-31-4 (Fibrin) |
| تواريخ الأحداث: | Date Created: 20170728 Date Completed: 20180724 Latest Revision: 20180724 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s10561-017-9645-2 |
| PMID: | 28748415 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-6814 |
|---|---|
| DOI: | 10.1007/s10561-017-9645-2 |