دورية أكاديمية
Validation of an HPLC-UV method for analysis of Kaempferol-loaded nanoemulsion and its application to in vitro and in vivo tests.
| العنوان: | Validation of an HPLC-UV method for analysis of Kaempferol-loaded nanoemulsion and its application to in vitro and in vivo tests. |
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| المؤلفون: | Colombo M; Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 90610-000, Porto Alegre, RS, Brazil., Melchiades GL; Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 90610-000, Porto Alegre, RS, Brazil., Figueiró F; Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Ramiro Barcelos, 2600, Anexo, 90035-003, Porto Alegre, RS, Brazil; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Ramiro Barcelos, 2600, Anexo, 90035-003, Porto Alegre, RS, Brazil., Battastini AMO; Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Ramiro Barcelos, 2600, Anexo, 90035-003, Porto Alegre, RS, Brazil; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Av. Ramiro Barcelos, 2600, Anexo, 90035-003, Porto Alegre, RS, Brazil., Teixeira HF; Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 90610-000, Porto Alegre, RS, Brazil., Koester LS; Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 90610-000, Porto Alegre, RS, Brazil. Electronic address: leticia.koester@ufrgs.br. |
| المصدر: | Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2017 Oct 25; Vol. 145, pp. 831-837. Date of Electronic Publication: 2017 Aug 05. |
| نوع المنشور: | Journal Article; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2006->: London : Elsevier Science Original Publication: Oxford ; New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid*, Animals ; Drug Delivery Systems ; Emulsions ; Kaempferols ; Mice ; Rats ; Reproducibility of Results |
| مستخلص: | A simple and reliable HPLC-UV method for Kaempferol (KPF) determination in a Kaempferol-loaded nanoemulsion (KPF-NE), samples from mucosa permeation/retention studies, and murine brain was developed and validated according to international guidelines. The analyses were performed on a reversed-phase C18 column at 35°C and under UV detection at 368nm. The mobile phase was composed of methanol:formic acid 0.1% (75:25, v/v) and was eluted at an isocratic flow rate of 1.0mL/min. The method was selective and sensitive for KPF analysis in matrix extracts, and linear in the range of 0.25-7.5μg/mL. The method was also considered precise, accurate, and robust. The recovery rates of KPF from the porcine nasal mucosa and murine brain were higher than 85%. Low matrix effect was observed to determine KPF, including biological matrices. The applicability of the method was confirmed in all different approaches, i.e., quantification of KPF in nanoemulsion, in vitro permeation/retention of KPF across porcine nasal mucosa, and in vivo quantification of KPF in brain samples after nasal administration in rats. Thus, the method is effective and reliable to determine KPF in different real samples. The proposed method, therefore, provides a useful quantification approach to routine processes, to the development of drug delivery systems, and to KPF quantification in different biological matrices. Furthermore, the method is applicable in bioavailability studies and the developed formulation (KPF-NE) is suitable for preclinical trials in different brain disorders. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Brain delivery; High-performance liquid chromatography; Kaempferol; Nanoemulsion; Permeation/retention assays; Validation |
| المشرفين على المادة: | 0 (Emulsions) 0 (Kaempferols) |
| تواريخ الأحداث: | Date Created: 20170823 Date Completed: 20180131 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jpba.2017.07.046 |
| PMID: | 28826141 |
| قاعدة البيانات: | MEDLINE |
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