دورية أكاديمية
Analysis of Prion Protein Conformation Using Circular Dichroism Spectroscopy.
| العنوان: | Analysis of Prion Protein Conformation Using Circular Dichroism Spectroscopy. |
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| المؤلفون: | Ellett LJ; Department of Pathology, The University of Melbourne, Parkville, Melbourne, VIC, 3010, Australia., Johanssen VA; Department of Medicine, The University of Melbourne, Parkville, Melbourne, VIC, 3010, Australia. vanessa.johanssen@oncology.ox.ac.uk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1658, pp. 27-34. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Protein Folding*, Circular Dichroism/*methods , Prion Proteins/*chemistry, Freeze Drying ; Humans ; Hydrogen-Ion Concentration ; Kinetics ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Recombinant Proteins/chemistry ; Temperature ; Thermodynamics |
| مستخلص: | According to the protein-only hypothesis of prion propagation, the pathogenesis of prion disease is due to the misfolding of cellular PrP (PrP C ) which gives rise to disease-associated PrP Sc . This misfolding results in the predominantly α-helix secondary structure of PrP becoming increasingly β-sheet. Prion protein researchers often employ circular dichroism (CD) spectroscopy to rapidly analyze and identify the degree of α-helix and β-sheet content in their recombinant protein and peptide samples. CD is a nondestructive method of determining protein secondary structure and can be used to monitor the protein structural changes in various environments, e.g., pH and temperature. CD can also be used to investigate kinetic and thermodynamic characteristics of proteins and peptides. |
| فهرسة مساهمة: | Keywords: CD; Circular dichroism; Misfolded; Protein conformation; Recombinant protein; Secondary structure; Spectra; Spectrometer; Spectroscopy; α-Helix; β-Sheet |
| المشرفين على المادة: | 0 (Prion Proteins) 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 20170902 Date Completed: 20180515 Latest Revision: 20180809 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-4939-7244-9_3 |
| PMID: | 28861780 |
| قاعدة البيانات: | MEDLINE |
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