دورية أكاديمية
Purine Biosynthesis Enzymes in Hippocampal Neurons.
| العنوان: | Purine Biosynthesis Enzymes in Hippocampal Neurons. |
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| المؤلفون: | Williamson J; Laboratory of Neurosciences, NIA/NIH, Baltimore, MD, 21224, USA., Petralia RS; Advanced Imaging Core, NIDCD/NIH, Bethesda, MD, 20892, USA., Wang YX; Advanced Imaging Core, NIDCD/NIH, Bethesda, MD, 20892, USA., Mattson MP; Laboratory of Neurosciences, NIA/NIH, Baltimore, MD, 21224, USA., Yao PJ; Laboratory of Neurosciences, NIA/NIH, Baltimore, MD, 21224, USA. Pamela.Yao@nih.gov. |
| المصدر: | Neuromolecular medicine [Neuromolecular Med] 2017 Dec; Vol. 19 (4), pp. 518-524. Date of Electronic Publication: 2017 Sep 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 101135365 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-1174 (Electronic) Linking ISSN: 15351084 NLM ISO Abbreviation: Neuromolecular Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Totowa, NJ : Humana Press, c2002- |
| مواضيع طبية MeSH: | Hippocampus/*metabolism , Neurons/*metabolism , Purines/*biosynthesis, Animals ; Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/analysis ; Cells, Cultured ; HeLa Cells ; Hippocampus/cytology ; Hippocampus/embryology ; Humans ; Hydroxymethyl and Formyl Transferases/analysis ; Microscopy, Electron ; Microscopy, Fluorescence ; Mitochondria/enzymology ; Multienzyme Complexes/analysis ; Nerve Tissue Proteins/analysis ; Neurons/enzymology ; Neurons/ultrastructure ; Nucleotide Deaminases/analysis ; Peptide Synthases/analysis ; Primary Cell Culture ; Rats ; Subcellular Fractions/enzymology |
| مستخلص: | Despite reports implicating disrupted purine metabolism in causing a wide spectrum of neurological defects, the mechanistic details of purine biosynthesis in neurons are largely unknown. As an initial step in filling that gap, we examined the expression and subcellular distribution of three purine biosynthesis enzymes (PFAS, PAICS and ATIC) in rat hippocampal neurons. Using immunoblotting and high-resolution light and electron microscopic analysis, we find that all three enzymes are broadly distributed in hippocampal neurons with pools of these enzymes associated with mitochondria. These findings suggest a potential link between purine metabolism and mitochondrial function in neurons and provide an impetus for further studies. |
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| معلومات مُعتمدة: | P50 DC000081 United States DC NIDCD NIH HHS; Z99 DC999999 United States ImNIH Intramural NIH HHS |
| فهرسة مساهمة: | Keywords: Hippocampus; Mitochondria; Neuron; Purine biosynthesis |
| المشرفين على المادة: | 0 (Multienzyme Complexes) 0 (Nerve Tissue Proteins) 0 (Purines) 0 (inosine monophosphate synthase) EC 2.1.2.- (Hydroxymethyl and Formyl Transferases) EC 3.5.4.- (Nucleotide Deaminases) EC 6.3.2.- (Peptide Synthases) EC 6.3.2.6 (phosphoribosylaminoimidazole-succinocarboxamide synthetase) EC 6.3.5.- (Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor) EC 6.3.5.3 (phosphoribosylformylglycinamidine synthetase) |
| تواريخ الأحداث: | Date Created: 20170904 Date Completed: 20180718 Latest Revision: 20240620 |
| رمز التحديث: | 20240620 |
| مُعرف محوري في PubMed: | PMC6085884 |
| DOI: | 10.1007/s12017-017-8466-6 |
| PMID: | 28866774 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1559-1174 |
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| DOI: | 10.1007/s12017-017-8466-6 |