دورية أكاديمية
Lessons from the Hamster: Cricetulus griseus Tissue and CHO Cell Line Proteome Comparison.
| العنوان: | Lessons from the Hamster: Cricetulus griseus Tissue and CHO Cell Line Proteome Comparison. |
|---|---|
| المؤلفون: | Heffner KM; Johns Hopkins University , Baltimore, Maryland 21218, United States., Hizal DB; Johns Hopkins University , Baltimore, Maryland 21218, United States., Yerganian GS; Brandeis University , Waltham, Massachusetts 02453, United States., Kumar A; Johns Hopkins University , Baltimore, Maryland 21218, United States., Can Ö; Acibadem University , Medical Biochemistry, Istanbul, Maltepe, Turkey., O'Meally R; Johns Hopkins Medical Institute , Baltimore, Maryland 21205, United States., Cole R; Johns Hopkins Medical Institute , Baltimore, Maryland 21205, United States., Chaerkady R; MedImmune , Gaithersburg, Maryland 20878, United States., Wu H; MedImmune , Gaithersburg, Maryland 20878, United States., Bowen MA; MedImmune , Gaithersburg, Maryland 20878, United States., Betenbaugh MJ; Johns Hopkins University , Baltimore, Maryland 21218, United States. |
| المصدر: | Journal of proteome research [J Proteome Res] 2017 Oct 06; Vol. 16 (10), pp. 3672-3687. Date of Electronic Publication: 2017 Sep 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
| مواضيع طبية MeSH: | Proteomics*, CHO Cells/*metabolism , Proteome/*genetics , Recombinant Proteins/*genetics, Animals ; Cricetinae ; Cricetulus/genetics ; Cricetulus/metabolism ; Recombinant Proteins/metabolism ; Tandem Mass Spectrometry |
| مستخلص: | Chinese hamster ovary cells represent the dominant host for therapeutic recombinant protein production. However, few large-scale data sets have been generated to characterize this host organism and derived CHO cell lines at the proteomics level. Consequently, an extensive label-free quantitative proteomics analysis of two cell lines (CHO-S and CHO DG44) and two Chinese hamster tissues (liver and ovary) was used to identify a total of 11 801 unique proteins containing at least two unique peptides. 9359 unique proteins were identified specifically in the cell lines, representing a 56% increase over previous work. Additionally, 6663 unique proteins were identified across liver and ovary tissues, providing the first Chinese hamster tissue proteome. Protein expression was more conserved within cell lines during both growth phases than across cell lines, suggesting large genetic differences across cell lines. Overall, both gene ontology and KEGG pathway analysis revealed enrichment of cell-cycle activity in cells. In contrast, upregulated molecular functions in tissue include glycosylation and lipid transporter activity. Furthermore, cellular components including Golgi apparatus are upregulated in both tissues. In conclusion, this large-scale proteomics analysis enables us to delineate specific changes between tissues and cells derived from these tissues, which can help explain specific tissue function and the adaptations cells incur for applications in biopharmaceutical productions. |
| فهرسة مساهمة: | Keywords: CHO; KEGG; cell culture; gene ontology; hamster tissue; liver; mass spectrometry; ovary; pathway analysis; proteomics |
| المشرفين على المادة: | 0 (Proteome) 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 20170907 Date Completed: 20180522 Latest Revision: 20181118 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1021/acs.jproteome.7b00382 |
| PMID: | 28876938 |
| قاعدة البيانات: | MEDLINE |
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