دورية أكاديمية
أعرض في EDS

The genetic diversity of metronidazole susceptibility in Trichomonas vaginalis clinical isolates in an Egyptian population.

التفاصيل البيبلوغرافية
العنوان: The genetic diversity of metronidazole susceptibility in Trichomonas vaginalis clinical isolates in an Egyptian population.
المؤلفون: Abdel-Magied AA; Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., El-Kholya EI; Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abou El-Khair SM; Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abdelmegeed ES; Department of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt., Hamoudaa MM; Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Mohamed SA; Department of Gynecology and Obstetrics, Mansoura University, Mansoura, Egypt., El-Tantawy NL; Department of Parasitology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. noratantawy@yahoo.com.
المصدر: Parasitology research [Parasitol Res] 2017 Nov; Vol. 116 (11), pp. 3125-3130. Date of Electronic Publication: 2017 Sep 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Springer International, c1987-
مواضيع طبية MeSH: Antiprotozoal Agents/*therapeutic use , Drug Resistance/*genetics , Metronidazole/*therapeutic use , Trichomonas Vaginitis/*drug therapy , Trichomonas vaginalis/*drug effects , Trichomonas vaginalis/*genetics, Adolescent ; Adult ; Egypt ; Female ; Genetic Variation/genetics ; Humans ; Middle Aged ; Parasitic Sensitivity Tests ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length/genetics ; Trichomonas Vaginitis/parasitology ; Trichomonas vaginalis/isolation & purification ; Vaginal Smears ; Young Adult
مستخلص: Trichomoniasis is the most common curable sexually transmitted disease worldwide. Resistance to metronidazole in treating trichomoniasis is a problematic health issue. We aimed to determine the minimum lethal concentration (MLC) of metronidazole for Trichomonas vaginalis isolates detected in Mansoura, Egypt and studied the genotypic profile of these isolates. Vaginal swab specimens were obtained from 320 symptomatic and 100 asymptomatic females, for whom clinical examination, vaginal discharge wet mount, Giemsa stain, and culture in modified Diamond's media were performed. Metronidazole susceptibility testing by an aerobic tube assay was performed. Both sensitive and resistant isolates were examined by PCR amplification followed by restriction fragment length polymorphism (RFLP). Trichomonas vaginalis was identified in 49/420 (11.7%) using either culture or PCR, while wet mount and Giemsa stain detected the parasite in 8.1 and 7.6% of participants, respectively. After 48 h incubation, most isolates were sensitive to metronidazole with a minimal lethal concentration (MLC) of 1 μg/ml. Mild resistance was observed in two isolates with MLCs of 64 μg\ml and mild to moderate resistance was observed in an additional two isolates with MLCs of 128 μg/ml. The four isolates that demonstrated low to moderate metronidazole resistance displayed a unique genotype band pattern by RFLP compared to the other 45 samples that were metronidazole sensitive. Our results highlight the presence of in vitro metronidazole tolerance in a few T. vaginalis isolates in Mansoura, Egypt that may lead to the development of drug resistance as well as the possibility of an identifying RFLP pattern in the isolates.
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فهرسة مساهمة: Keywords: MLC; Metronidazole; PCR-RFLP; Susceptibility; T. vaginalis
المشرفين على المادة: 0 (Antiprotozoal Agents)
140QMO216E (Metronidazole)
تواريخ الأحداث: Date Created: 20170929 Date Completed: 20180313 Latest Revision: 20181202
رمز التحديث: 20231215
DOI: 10.1007/s00436-017-5627-3
PMID: 28956167
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-1955
DOI:10.1007/s00436-017-5627-3