دورية أكاديمية
أعرض في EDS

Characterization of the Zika virus induced small RNA response in Aedes aegypti cells.

التفاصيل البيبلوغرافية
العنوان: Characterization of the Zika virus induced small RNA response in Aedes aegypti cells.
المؤلفون: Varjak M; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom., Donald CL; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom., Mottram TJ; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom., Sreenu VB; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom., Merits A; Institute of Technology, University of Tartu, Nooruse 1, Tartu, Estonia., Maringer K; Department of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom., Schnettler E; Bernhard-Nocht-Institute for Tropical Medicine, Bernhard-Nocht-Strasse, Hamburg, Germany., Kohl A; MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2017 Oct 17; Vol. 11 (10), pp. e0006010. Date of Electronic Publication: 2017 Oct 17 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Host-Pathogen Interactions* , RNA Interference* , RNA, Small Interfering*, Aedes/*virology , Insect Proteins/*genetics , Zika Virus/*physiology, Aedes/cytology ; Alphavirus/genetics ; Animals ; Capsid Proteins/genetics ; Cell Line ; Insect Proteins/metabolism ; Virus Replication ; Yellow fever virus/genetics ; Zika Virus/genetics ; Zika Virus Infection/transmission
مستخلص: RNA interference (RNAi) controls arbovirus infections in mosquitoes. Two different RNAi pathways are involved in antiviral responses: the PIWI-interacting RNA (piRNA) and exogenous short interfering RNA (exo-siRNA) pathways, which are characterized by the production of virus-derived small RNAs of 25-29 and 21 nucleotides, respectively. The exo-siRNA pathway is considered to be the key mosquito antiviral response mechanism. In Aedes aegypti-derived cells, Zika virus (ZIKV)-specific siRNAs were produced and loaded into the exo-siRNA pathway effector protein Argonaute 2 (Ago2); although the knockdown of Ago2 did not enhance virus replication. Enhanced ZIKV replication was observed in a Dcr2-knockout cell line suggesting that the exo-siRNA pathway is implicated in the antiviral response. Although ZIKV-specific piRNA-sized small RNAs were detected, these lacked the characteristic piRNA ping-pong signature motif and were bound to Ago3 but not Piwi5 or Piwi6. Silencing of PIWI proteins indicated that the knockdown of Ago3, Piwi5 or Piwi6 did not enhance ZIKV replication and only Piwi4 displayed antiviral activity. We also report that the expression of ZIKV capsid (C) protein amplified the replication of a reporter alphavirus; although, unlike yellow fever virus C protein, it does not inhibit the exo-siRNA pathway. Our findings elucidate ZIKV-mosquito RNAi interactions that are important for understanding its spread.
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معلومات مُعتمدة: 096062 United Kingdom Wellcome Trust; MC_UU_12014/12 United Kingdom MRC_ Medical Research Council; MC_UU_12014/8 United Kingdom MRC_ Medical Research Council; G0801822 United Kingdom MRC_ Medical Research Council; United Kingdom Wellcome Trust
المشرفين على المادة: 0 (Capsid Proteins)
0 (Insect Proteins)
0 (RNA, Small Interfering)
تواريخ الأحداث: Date Created: 20171018 Date Completed: 20171108 Latest Revision: 20220129
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5667879
DOI: 10.1371/journal.pntd.0006010
PMID: 29040304
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0006010