دورية أكاديمية
Comparison of the COBAS/Ampliprep Taqman and Amplicor HIV-1 monitor tests in Lagos, Nigeria.
| العنوان: | Comparison of the COBAS/Ampliprep Taqman and Amplicor HIV-1 monitor tests in Lagos, Nigeria. |
|---|---|
| المؤلفون: | Amoo OS; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Taiwo IA; Department of Cell Biology & Genetics, University of Lagos, Akoka, Lagos, Nigeria., Salu OO; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Okwuraiwe AP; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Onwuamah CK; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Awe MA; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Oforomeh OO; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Onwujekwe DI; Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Ezechi OC; Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria., Ajuma AR; Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria. |
| المصدر: | African journal of laboratory medicine [Afr J Lab Med] 2013 May 15; Vol. 2 (1), pp. 68. Date of Electronic Publication: 2013 May 15 (Print Publication: 2013). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: AOSIS OpenJournals Country of Publication: South Africa NLM ID: 101603205 Publication Model: eCollection Cited Medium: Print ISSN: 2225-2002 (Print) Linking ISSN: 22252002 NLM ISO Abbreviation: Afr J Lab Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Durbanville, South Africa : AOSIS OpenJournals, [2012]- |
| مستخلص: | Background: The use of real-time Polymerase chain reaction (PCR) technology options is increasing in resource-limited settings because they are faster, improve assay sensitivity, have higher throughput, larger dynamic ranges and reduced rates of contamination. In 2010, UNAIDS ranked Nigeria as the second highest population of people living with HIV and AIDS (2.98 million people) in the world. Objective: The objective of this study was to compare the analytical performances of the Amplicor HIV-1 Monitor (version 1.5) and the COBAS Ampliprep/Taqman (version 2.0) used in monitoring HIV disease progression in HIV-infected individuals. Method: In a cross-sectional study, HIV-1 RNA values obtained with the Amplicor HIV-1 monitor version 1.5 were compared with those of the COBAS/Ampliprep TaqMan HIV-1 version 2.0 in a routine clinical setting. Between May and November 2011, 176 plasma samples collected were analysed in parallel using both techniques. Data analysis was done using statgraphics Centurion XVI and Medcalc version 12.0. Result: The correlation coefficient for the two assays was 0.83 and the level of agreement using a Bland-Altman plot was 94.2%. Conclusion: These findings suggest that the results from the two methods were comparable, hence the COBAS/Ampliprep Taqman version 2.0 is recommended for high-volume laboratories. Competing Interests: The authors declare that they have no financial or personal relationship(s) which may have inappropriately influenced them in writing this article. |
| References: | J Clin Microbiol. 2010 Apr;48(4):1337-42. (PMID: 20164284) PLoS Med. 2006 Dec;3(12):e540. (PMID: 17194205) Rev Inst Med Trop Sao Paulo. 2006 Mar-Apr;48(2):77-80. (PMID: 16699628) J Clin Microbiol. 2006 Aug;44(8):2963-6. (PMID: 16891517) Stat Methods Med Res. 1999 Jun;8(2):135-60. (PMID: 10501650) Clin Infect Dis. 2011 Dec;53(12):1283-90. (PMID: 22080121) AIDS. 2005 Jul 1;19(10):995-1005. (PMID: 15958830) J Clin Virol. 2005 May;33(1):43-51. (PMID: 15797364) J Clin Microbiol. 2009 Oct;47(10):3400-2. (PMID: 19656975) AIDS Res Hum Retroviruses. 2000 Mar 1;16(4):315-25. (PMID: 10716369) Intervirology. 2010;53(4):221-8. (PMID: 20332651) J Clin Microbiol. 2007 Oct;45(10):3436-8. (PMID: 17715371) J Acquir Immune Defic Syndr. 2009 Oct 1;52(2):228-34. (PMID: 19644383) |
| تواريخ الأحداث: | Date Created: 20171019 Latest Revision: 20201001 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC5637769 |
| DOI: | 10.4102/ajlm.v2i1.68 |
| PMID: | 29043166 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!