دورية أكاديمية
General Staining and Segmentation Procedures for High Content Imaging and Analysis.
| العنوان: | General Staining and Segmentation Procedures for High Content Imaging and Analysis. |
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| المؤلفون: | Chambers KM; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA., Mandavilli BS; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA. Bhaskar.mandavilli@thermofisher.com., Dolman NJ; Thermo Fisher Scientific, 100 Technology Drive, Pittsburgh, PA, 15219, USA., Janes MS; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1683, pp. 21-31. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | High-Throughput Screening Assays* , Image Processing, Computer-Assisted* , Molecular Imaging*/methods , Staining and Labeling*/methods, Animals ; Cell Line ; Cell Nucleus ; Fluorescent Dyes ; Humans |
| مستخلص: | Automated quantitative fluorescence microscopy, also known as high content imaging (HCI), is a rapidly growing analytical approach in cell biology. Because automated image analysis relies heavily on robust demarcation of cells and subcellular regions, reliable methods for labeling cells is a critical component of the HCI workflow. Labeling of cells for image segmentation is typically performed with fluorescent probes that bind DNA for nuclear-based cell demarcation or with those which react with proteins for image analysis based on whole cell staining. These reagents, along with instrument and software settings, play an important role in the successful segmentation of cells in a population for automated and quantitative image analysis. In this chapter, we describe standard procedures for labeling and image segmentation in both live and fixed cell samples. The chapter will also provide troubleshooting guidelines for some of the common problems associated with these aspects of HCI. |
| فهرسة مساهمة: | Keywords: CellMask; CellTracker; High content imaging; High content screening; Nuclear segmentation; Segmentation |
| المشرفين على المادة: | 0 (Fluorescent Dyes) |
| تواريخ الأحداث: | Date Created: 20171031 Date Completed: 20180619 Latest Revision: 20180904 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-4939-7357-6_2 |
| PMID: | 29082484 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-7357-6_2 |