دورية أكاديمية
Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis.
| العنوان: | Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis. |
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| المؤلفون: | Mandavilli BS; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA. Bhaskar.mandavilli@thermofisher.com., Aggeler RJ; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA., Chambers KM; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1683, pp. 33-46. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | High-Throughput Screening Assays* , Image Processing, Computer-Assisted* , Molecular Imaging*/methods, Animals ; Cell Line ; Cell Survival ; Drug Discovery ; Humans ; Lipid Metabolism ; Membrane Potential, Mitochondrial/drug effects ; Mitochondria/drug effects ; Mitochondria/metabolism ; Oxidative Stress ; Phospholipids/metabolism ; Reactive Oxygen Species/metabolism ; Toxicity Tests |
| مستخلص: | High content screening (HCS)-based multiparametric measurements are very useful in early toxicity testing and safety assessment during drug development, and useful in evaluating the impact from new food supplements and environmental toxicants. Mitochondrial membrane potential, plasma membrane permeability, oxidative stress, phosphoplipidosis, and steatosis are a few of the important markers routinely studied for the assessment of drug-induced liver injury and toxicity. Mitochondrial dysfunction leads to oxidative stress and cell death. Liver injury from drug-induced phospholipidosis and steatosis is routinely studied in hepatotoxicity investigations to determine the risk factors and fate of drugs or chemical compounds as some drugs can lead to defects in lipid metabolism and accumulation of lipids in lysosomes. In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma membrane permeability, oxidative stress, phospholipidosis, and steatosis using high content imaging-based methodologies and instrumentation. |
| فهرسة مساهمة: | Keywords: Cytotoxicity; Mitochondrial membrane potential; Oxidative stress; Plasma membrane integrity; Reactive oxygen species |
| المشرفين على المادة: | 0 (Phospholipids) 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20171031 Date Completed: 20180619 Latest Revision: 20180904 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1007/978-1-4939-7357-6_3 |
| PMID: | 29082485 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-7357-6_3 |