دورية أكاديمية
Tools to Measure Autophagy Using High Content Imaging and Analysis.
| العنوان: | Tools to Measure Autophagy Using High Content Imaging and Analysis. |
|---|---|
| المؤلفون: | Dolman NJ; Thermo Fisher Scientific, 100 Technology Drive, Pittsburgh, PA, 15219, USA., Samson BA; Thermo Fisher Scientific, 100 Technology Drive, Pittsburgh, PA, 15219, USA., Chambers KM; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA., Janes MS; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA., Mandavilli BS; Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA. Bhaskar.mandavilli@thermofisher.com. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1683, pp. 59-71. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Autophagy* , High-Throughput Screening Assays* , Image Processing, Computer-Assisted* , Molecular Imaging*/methods, Animals ; Biomarkers ; CRISPR-Cas Systems ; Cells, Cultured ; Fluorescent Antibody Technique ; Gene Editing ; Gene Knockout Techniques ; Genes, Reporter ; Humans ; Mice ; Microtubule-Associated Proteins/metabolism ; Phagosomes/metabolism ; Rats |
| مستخلص: | Macroautophagy, hereafter referred to as autophagy, is a predominately pro-survival catabolic process responsible for the degradation of long-lived or aggregated proteins, invading microorganisms and damaged or redundant intracellular organelles. Removal of these entities is achieved through encompassment of the target by the autophagosome and subsequent delivery to the lysosome. The use of fluorescence microscopy is a common method to investigate autophagy through monitoring the spatial and temporal recruitment both of autophagosomal markers and cargo to the autophagosome. In this section, we will discuss the use of high content imaging (HCI) and analysis in the study of autophagy with reference to commonly used markers of autophagosomal formation. |
| فهرسة مساهمة: | Keywords: ATG5; Autophagosome; Autophagy; CRISPR; High content imaging; High content screening; LC3B |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Microtubule-Associated Proteins) |
| تواريخ الأحداث: | Date Created: 20171031 Date Completed: 20180619 Latest Revision: 20180904 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-4939-7357-6_5 |
| PMID: | 29082487 |
| قاعدة البيانات: | MEDLINE |
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