دورية أكاديمية
Development of a Screening Method and Device for the Detection of Escherichia coli from Agri-Food Production Environments and Fresh Produce.
| العنوان: | Development of a Screening Method and Device for the Detection of Escherichia coli from Agri-Food Production Environments and Fresh Produce. |
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| المؤلفون: | Yun B; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., An HM; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., Shim WB; Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, Jinju 52828, Republic of Korea., Kim WI; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., Hung NB; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., Han S; Division of Research Policy, Research Policy Bureau, Rural Development Administration, Jeonju 54875, Republic of Korea., Kim HJ; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., Lee S; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea., Kim SR; Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institution of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea. |
| المصدر: | Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2017 Dec 28; Vol. 27 (12), pp. 2141-2150. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print Cited Medium: Internet ISSN: 1738-8872 (Electronic) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001. |
| مواضيع طبية MeSH: | Escherichia coli O157/*isolation & purification , Food Microbiology/*methods , Microbiological Techniques/*instrumentation , Vegetables/*microbiology, Agriculture ; Colony Count, Microbial ; Incubators ; Limit of Detection ; Temperature ; Ultraviolet Rays |
| مستخلص: | This study was conducted to develop a screening method using Colilert-18 and a device for the detection of E. coli from agri-food production environments and fresh vegetables. The specificity and sensitivity of Colilert-18 by temperature (37°C and 44°C) were evaluated with 38 E. coli and 78 non- E. coli strains. The false-positive rate was 3.8% (3/78) and 0% (0/78) at 37°C and 44°C, respectively. The detection limit of E. coli at 37°C at <1.0 log CFU/250 ml was lower than that at 44°C. The efficiency of the developed device, which comprised an incubator equipped with a UV lamp to detect E. coli in the field, was evaluated by measuring the temperature and UV lamp brightness. The difference between the set temperature and actual temperature of the developed device was about 1.0°C. When applying the developed method and device to various samples, including utensils, gloves, irrigation water, seeds, and vegetables, there were no differences in detection rates of E. coli compared with the Korean Food Code method. For sanitary disposal of culture samples after experiments, the sterilization effect of sodium dichloroisocyanurate (NaDCC) tablets was assessed for use as a substitute for an autoclave. The addition of one tablet of NaDCC per 50 ml was sufficient to kill E. coli cultured in Colilert-18. These results show that the developed protocol and device can efficiently detect E. coli from agri-food production environments and vegetables. |
| فهرسة مساهمة: | Keywords: Colilert-18; E. coli; NaDCC; device; on-site detection |
| تواريخ الأحداث: | Date Created: 20171103 Date Completed: 20190219 Latest Revision: 20190320 |
| رمز التحديث: | 20231215 |
| DOI: | 10.4014/jmb.1709.09019 |
| PMID: | 29092389 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1738-8872 |
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| DOI: | 10.4014/jmb.1709.09019 |