دورية أكاديمية
أعرض في EDS

Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate.

التفاصيل البيبلوغرافية
العنوان: Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate.
المؤلفون: Yuzhakov AG; Laboratory of Virology, Diagnostics and Prevention Research Institute for Human and Animal Diseases, Moscow, Russia. Electronic address: anton_oskol@mail.ru., Raev SA; Laboratory of Virology, Diagnostics and Prevention Research Institute for Human and Animal Diseases, Moscow, Russia. Electronic address: raevsergey@mail.ru., Skrylev AN; Siberian Agrarian Group, Tomsk, Russia. Electronic address: shan.70@yandex.ru., Mishin AM; Laboratory of Virology, Diagnostics and Prevention Research Institute for Human and Animal Diseases, Moscow, Russia. Electronic address: doktor-mishin@mail.ru., Grebennikova TV; Laboratory of Virology, Diagnostics and Prevention Research Institute for Human and Animal Diseases, Moscow, Russia. Electronic address: t_grebennikova@mail.ru., Verkhovsky OA; Laboratory of Virology, Diagnostics and Prevention Research Institute for Human and Animal Diseases, Moscow, Russia. Electronic address: info@dpri.ru., Zaberezhny AD; All-Russian Research Institute of Experimental Veterinary Medicine named Ya. R. Kovalenko, Moscow, Russia. Electronic address: zaberezhny@mail.ru., Trus I; Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Electronic address: ivan.trus@ugent.be., Nauwynck HJ; Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Electronic address: hans.nauwynck@ugent.be., Aliper TI; All-Russian Research Institute of Experimental Veterinary Medicine named Ya. R. Kovalenko, Moscow, Russia. Electronic address: aliper@narvac.com.
المصدر: Veterinary microbiology [Vet Microbiol] 2017 Nov; Vol. 211, pp. 22-28. Date of Electronic Publication: 2017 Sep 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier Scientific Pub. Co.
مواضيع طبية MeSH: Genome, Viral/*genetics , Porcine Reproductive and Respiratory Syndrome/*virology , Porcine respiratory and reproductive syndrome virus/*pathogenicity , Viremia/*veterinary, Amino Acid Sequence ; Animals ; Lung/virology ; Phylogeny ; Porcine respiratory and reproductive syndrome virus/genetics ; Porcine respiratory and reproductive syndrome virus/isolation & purification ; Random Allocation ; Russia ; Sequence Alignment/veterinary ; Swine ; Viral Load/veterinary ; Viremia/virology ; Virulence
مستخلص: Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory problems. Data about the virulence and pathogenicity of subtype 2 PRRSV-1 strains are limited. The main purposes of this investigation were to characterize the full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain and to compare the pathogenicity with that of the subtype 1 PRRSV-1 Lelystad strain. Comparison of the whole genome sequence of the WestSib13 strain with that of PRRSV-1 prototype strains revealed a 76.2% (subtype 1 Lelystad virus) and 79.0% (subtype 3 Lena virus) identity, respectively The virulence and pathogenicity of the European subtype 2 PRRSV strain WestSib13 and the European subtype 1 PRRSV strain Lelystad were compared in 3-week-old piglets upon inoculation of 10 5.4 TCID 50 of virus. Non-infected animals (control group) as well as animals infected with the Lelystad strain were clinically healthy until 14days post challenge. In contrast, animals infected with the WestSib13 strain demonstrated dyspnea starting at 3days post-inoculation (dpi). All piglets in this group died between 5 and 8 dpi. During that period, fever was not observed in WestSib13-infected animals. Viremia was detected in animals from both infected groups starting from 2 dpi. Viral loads in serum and lungs upon euthanasia were significantly higher (3 log 10 ) in the WestSib13-infected than in the LV-infected animals. Taken together, this study provides the full genome sequence and the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Fever; Genome; Mortality; PRRSV; Pathogenicity; Subtype 2; Virulence
تواريخ الأحداث: Date Created: 20171106 Date Completed: 20180702 Latest Revision: 20180702
رمز التحديث: 20240628
DOI: 10.1016/j.vetmic.2017.09.017
PMID: 29102117
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2542
DOI:10.1016/j.vetmic.2017.09.017