دورية أكاديمية
In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis.
| العنوان: | In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis. |
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| المؤلفون: | Estell C; Institute of Cancer Science, Glasgow University, Glasgow G61 1QH, UK., Stamatidou E; School of Medicine, Dentistry and Nursing, Glasgow University, Glasgow G12 8QQ, UK., El-Messeiry S; School of Medicine, Dentistry and Nursing, Glasgow University, Glasgow G12 8QQ, UK.; Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt., Hamilton A; School of Medicine, Dentistry and Nursing, Glasgow University, Glasgow G12 8QQ, UK andrew.hamilton@glasgow.ac.uk. |
| المصدر: | Journal of cell science [J Cell Sci] 2017 Dec 15; Vol. 130 (24), pp. 4193-4199. Date of Electronic Publication: 2017 Nov 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | Mitochondria/*genetics , Mitochondrial Proteins/*genetics , Mitosis/*genetics , Protein Biosynthesis/*genetics, Animals ; Click Chemistry ; DNA, Mitochondrial/genetics ; Genome, Mitochondrial/genetics ; Humans ; MCF-7 Cells ; Mice ; Microscopy, Fluorescence ; Mitochondria/ultrastructure |
| مستخلص: | Although mitochondrial translation produces only 13 proteins, we show here how this process can be visualised and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using non-canonical amino acid labelling and click chemistry. This allows visualisation of the translational output in different mitochondria within a cell, their position within that cell and a comparison of mitochondrial translation between cells. The most highly translationally active mitochondria were closest to the nucleus but were also found at the distal end of long cellular projections. There were substantial differences in translation between adjacent mitochondria and this did not readily correlate with apparent mitochondrial genome content. Mitochondrial translation was unchanged during mitosis when cytoplasmic translation was suppressed. This method will serve both fundamental cell biology and clinically orientated studies, in which mitochondrial function is a key parameter. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2017. Published by The Company of Biologists Ltd.) |
| فهرسة مساهمة: | Keywords: Click; Fluorescence; Labelling; Mitochondria; Translation |
| المشرفين على المادة: | 0 (DNA, Mitochondrial) 0 (Mitochondrial Proteins) |
| تواريخ الأحداث: | Date Created: 20171111 Date Completed: 20180720 Latest Revision: 20180720 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1242/jcs.206714 |
| PMID: | 29122981 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
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| DOI: | 10.1242/jcs.206714 |