دورية أكاديمية
أعرض في EDS

Comparison of Different Methods of Purification and Concentration in Production of Influenza Vaccine.

التفاصيل البيبلوغرافية
العنوان: Comparison of Different Methods of Purification and Concentration in Production of Influenza Vaccine.
المؤلفون: Asanzhanova NN; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan. anurika@mail.ru., Ryskeldinova SZ; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan., Chervyakova OV; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan., Khairullin BM; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan., Kasenov MM; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan., Tabynov KK; Research Institute of Biological Safety Problems, Zhambyl Region, Gvardeiskii settlement, Kazakhstan.
المصدر: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Dec; Vol. 164 (2), pp. 229-232. Date of Electronic Publication: 2017 Nov 25.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Springer
Original Publication: New York, Consultants Bureau.
مواضيع طبية MeSH: Chromatography, Agarose/*methods , Influenza Vaccines/*isolation & purification , Ovalbumin/*isolation & purification , Ultracentrifugation/*methods , Ultrafiltration/*methods, Amniotic Fluid/chemistry ; Amniotic Fluid/virology ; Animals ; Chick Embryo ; Humans ; Influenza A Virus, H1N1 Subtype/growth & development ; Influenza A Virus, H1N1 Subtype/immunology ; Influenza Vaccines/biosynthesis ; Influenza, Human/immunology ; Influenza, Human/prevention & control ; Influenza, Human/virology
مستخلص: The overwhelming majority of influenza vaccines are prepared with the use of chicken embryo allantoic fluid. The presence of ovalbumin (this protein constitutes >60% total protein in the allantoic fluid) in the vaccine can lead to severe allergy. Hence, effective reduction of ovalbumin content is of crucial importance for vaccine production. We compared two methods of purification and concentration of influenza virus: zonal gradient ultracentrifugation and combined ultrafiltration/diafiltration and exclusion chromatography protocol, used for fabrication of seasonal vaccines. Combined chromatography is comparable with zonal centrifugation protocol by the results of ovalbumin removal (to meet standard requirements).
فهرسة مساهمة: Keywords: chromatography; influenza; purification and concentration; ultracentrifugation; vaccine
المشرفين على المادة: 0 (Influenza Vaccines)
9006-59-1 (Ovalbumin)
تواريخ الأحداث: Date Created: 20171128 Date Completed: 20180716 Latest Revision: 20180716
رمز التحديث: 20240628
DOI: 10.1007/s10517-017-3964-y
PMID: 29177903
قاعدة البيانات: MEDLINE
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