دورية أكاديمية
5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporin.
العنوان: | 5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporin. |
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المؤلفون: | Lund K; Unit for Cell Signaling, Institute of Microbiology, Rikshospitalet, 0372, Oslo, Norway. Kaja.Lund@rr-research.no.; Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PO Box 1112, Blindern, 0317, Oslo, Norway. Kaja.Lund@rr-research.no., Olsen CE; Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, 0379, Oslo, Norway., Wong JJW; Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, 0379, Oslo, Norway., Olsen PA; Unit for Cell Signaling, Institute of Microbiology, Rikshospitalet, 0372, Oslo, Norway.; Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PO Box 1112, Blindern, 0317, Oslo, Norway., Solberg NT; Unit for Cell Signaling, Institute of Microbiology, Rikshospitalet, 0372, Oslo, Norway.; Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PO Box 1112, Blindern, 0317, Oslo, Norway., Høgset A; PCI Biotech AS, Ullernchaussèn 64, 0379, Oslo, Norway., Krauss S; Unit for Cell Signaling, Institute of Microbiology, Rikshospitalet, 0372, Oslo, Norway.; Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, PO Box 1112, Blindern, 0317, Oslo, Norway., Selbo PK; Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, 0379, Oslo, Norway. selbo@rr-research.no. |
المصدر: | Journal of experimental & clinical cancer research : CR [J Exp Clin Cancer Res] 2017 Dec 19; Vol. 36 (1), pp. 187. Date of Electronic Publication: 2017 Dec 19. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 8308647 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-9966 (Electronic) Linking ISSN: 03929078 NLM ISO Abbreviation: J Exp Clin Cancer Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2009- : London : BioMed Central Original Publication: [Roma] : APSIT, |
مواضيع طبية MeSH: | Adenocarcinoma/*pathology , Immunotoxins/*pharmacology , Pancreatic Neoplasms/*pathology , Photosensitizing Agents/*pharmacology , Porphyrins/*pharmacology, Antineoplastic Agents ; Autophagy/drug effects ; Cell Line, Tumor ; Drug Resistance, Neoplasm/drug effects ; Endoglin/antagonists & inhibitors ; Epithelial-Mesenchymal Transition ; Fluorouracil ; Humans ; Phototherapy/methods ; Ribosome Inactivating Proteins, Type 1/pharmacology ; Saporins |
مستخلص: | Background: Development of resistance to 5-fluorouracil (5-FU) is a major problem in treatment of various cancers including pancreatic cancer. In this study, we reveal important resistance mechanisms and photochemical strategies to overcome 5-FU resistance in pancreatic adenocarcinoma. Methods: 5-FU resistant (5-FUR), epithelial-to-mesenchymal-like sub-clones of the wild type pancreatic cancer cell line Panc03.27 were previously generated in our lab. We investigated the cytotoxic effect of the endosomal/lysosomal-localizing photosensitizer TPCS Results: The 5-FUR cell lines display hypersensitivity to PCT, which was linked to increased uptake of TPCS Conclusion: Our findings suggested that autophagy is an important resistance mechanism against the chemotherapeutic drug 5-FU in pancreatic cancer cells, and that inhibition of the autophagy process, either by CQ or lysosomal photodamage, can contribute to increased sensitivity to 5-FU. For the first time, we demonstrate the promise of PCI-based targeting of CD105 in site-specific elimination of 5-FU resistant pancreatic cancer cells in vitro. In conclusion, PCI-based targeting of CD105 may represent a potent anticancer strategy and should be further evaluated in pre-clinical models. |
References: | J Control Release. 2010 Nov 20;148(1):2-12. (PMID: 20600406) Br J Cancer. 2014 Apr 2;110(7):1698-704. (PMID: 24569464) J Control Release. 2015 May 28;206:37-48. (PMID: 25758331) Cell Death Differ. 2005 Nov;12 Suppl 2:1509-18. (PMID: 16247498) Trends Cell Biol. 2003 Mar;13(3):137-45. (PMID: 12628346) Pancreas. 2013 Nov;42(8):1283-90. (PMID: 24308064) J Cell Biol. 2007 Jul 30;178(3):437-51. (PMID: 17646396) Int J Clin Exp Pathol. 2015 May 01;8(5):5105-12. (PMID: 26191205) Int J Cancer. 2001 Jun 1;92(5):761-6. (PMID: 11340584) PLoS One. 2015 Apr 10;10(4):e0123684. (PMID: 25860483) Lancet Oncol. 2016 Sep;17(9):1217-29. (PMID: 27475428) Crit Rev Eukaryot Gene Expr. 2011;21(1):71-100. (PMID: 21967333) Eukaryot Cell. 2002 Feb;1(1):11-21. (PMID: 12455967) Cell Biosci. 2014 Mar 03;4(1):10. (PMID: 24581180) J Transl Med. 2004 Jun 11;2(1):18. (PMID: 15193152) J Cell Sci. 2004 Jun 1;117(Pt 13):2805-12. (PMID: 15169837) Stem Cells. 2010 Aug;28(8):1435-45. (PMID: 20572012) N Engl J Med. 2014 Sep 11;371(11):1039-49. (PMID: 25207767) PLoS One. 2013;8(2):e56679. (PMID: 23441212) J Biol Chem. 1991 Nov 15;266(32):21327-30. (PMID: 1939168) Autophagy. 2010 Jan;6(1):7-18. (PMID: 19855190) Annu Rev Pharmacol Toxicol. 2005;45:51-88. (PMID: 15822171) Clin Genitourin Cancer. 2017 Feb;15(1):77-85. (PMID: 27328856) Transl Oncol. 2017 Apr;10(2):184-189. (PMID: 28182993) Biomol Ther (Seoul). 2017 May 1;25(3):315-320. (PMID: 27737524) Dev Biol. 2007 Apr 1;304(1):420-32. (PMID: 17250821) J Photochem Photobiol B. 2001 Sep 15;62(3):146-52. (PMID: 11566278) Lasers Med Sci. 2003;18(3):128-33. (PMID: 14505195) Oncologist. 2019 Feb;24(2):202-210. (PMID: 30190302) BMC Cancer. 2012 Sep 27;12:434. (PMID: 23017148) Oncotarget. 2015 Jan 20;6(2):1143-56. (PMID: 25544758) Clin Cancer Res. 2005 Dec 1;11(23):8476-85. (PMID: 16322311) Photochem Photobiol Sci. 2011 Oct;10(10):1637-51. (PMID: 21773635) J Control Release. 2013 Jun 28;168(3):317-26. (PMID: 23567040) J Pharmacol Exp Ther. 2006 Nov;319(2):604-12. (PMID: 16902053) Free Radic Res. 2010 May;44(5):479-96. (PMID: 20370557) EBioMedicine. 2017 Feb;15:90-99. (PMID: 28057438) Eur J Biochem. 2000 Sep;267(17):5550-60. (PMID: 10951214) J Biol Chem. 1992 Sep 25;267(27):19027-30. (PMID: 1326540) World J Gastroenterol. 2002 Dec;8(6):1029-34. (PMID: 12439919) Cell Death Dis. 2014 Jan 09;5:e983. (PMID: 24407236) Clin Cancer Res. 2012 Sep 1;18(17):4820-9. (PMID: 22767667) CA Cancer J Clin. 2011 Jul-Aug;61(4):250-81. (PMID: 21617154) Gut. 2002 Apr;50(4):549-57. (PMID: 11889078) |
معلومات مُعتمدة: | 33445 Kreftforeningen; 2016023 Helse Sør-Øst RHF; 2017068 Helse Sør-Øst RHF; FU0803 The Norwegian Radium Hospital Research Foundation; SE1603 The Norwegian Radium Hospital Research Foundation |
فهرسة مساهمة: | Keywords: 5-FU resistance; Autophagy; CD105; Endoglin; Pancreatic cancer; Photochemical internalization |
المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (ENG protein, human) 0 (Endoglin) 0 (Immunotoxins) 0 (Photosensitizing Agents) 0 (Porphyrins) 0 (Ribosome Inactivating Proteins, Type 1) 0 (meso-tetraphenyl chlorin disulphonate) EC 3.2.2.22 (Saporins) U3P01618RT (Fluorouracil) |
تواريخ الأحداث: | Date Created: 20171221 Date Completed: 20180905 Latest Revision: 20231112 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC5738190 |
DOI: | 10.1186/s13046-017-0662-6 |
PMID: | 29258566 |
قاعدة البيانات: | MEDLINE |
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