دورية أكاديمية
Photodynamic therapy of mouse tumor model using chlorin e6- polyvinyl alcohol complex.
| العنوان: | Photodynamic therapy of mouse tumor model using chlorin e6- polyvinyl alcohol complex. |
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| المؤلفون: | Gavrina AI; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation; Lobachevsky State University, 603000 Nizhny Novgorod, Russian Federation. Electronic address: niibmt@nizhgma.ru., Shirmanova MV; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation., Aksenova NA; Semenov Institute of Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russian Federation; Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russian Federation., Yuzhakova DV; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation., Snopova LB; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation., Solovieva AB; Semenov Institute of Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russian Federation., Тimashev PS; Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russian Federation; Institute of Photonic Technologies, Research center 'Crystallography and Photonics', Russian Academy of Sciences, 142190 Troitsk, Moscow, Russian Federation., Dudenkova VV; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation; Lobachevsky State University, 603000 Nizhny Novgorod, Russian Federation., Zagaynova EV; Nizhny Novgorod State Medical Academy, 603005 Nizhny Novgorod, Russian Federation. |
| المصدر: | Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2018 Jan; Vol. 178, pp. 614-622. Date of Electronic Publication: 2017 Dec 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Elsevier Sequoia, 1987- |
| مواضيع طبية MeSH: | Photosensitizing Agents/*chemistry , Polyvinyl Alcohol/*chemistry , Porphyrins/*chemistry, Animals ; Cell Line, Tumor ; Cell Survival/drug effects ; Chlorophyllides ; Disease Models, Animal ; Mice ; Mice, Inbred BALB C ; Microscopy, Atomic Force ; Microscopy, Confocal ; Neoplasms/drug therapy ; Neoplasms/pathology ; Oxidation-Reduction ; Photochemotherapy ; Photosensitizing Agents/therapeutic use ; Photosensitizing Agents/toxicity ; Reactive Oxygen Species ; Spectrometry, Fluorescence ; Transplantation, Homologous ; Tryptophan/chemistry ; Whole Body Imaging |
| مستخلص: | The use of polymeric carriers to deliver hydrophobic photosensitizers has been widely discussed as a way to improve both fluorescence diagnostic and photodynamic therapy (PDT) of cancers; however, the photophysical and pharmacokinetic parameters, as well as the PDT activity, of such modifications have, until now, only been poorly investigated. The purpose of the present study was to explore the efficacy of PDT with the formulation of the photosensitizer chlorin e6 (Ce6) in combination with polyvinyl alcohol (PVA) in comparison with Ce6 alone and with the clinical drug, Photodithazine in a mouse tumor model. We also investigated the photoactivity of the Ce6-PVA in a model reaction of tryptophan oxidation, analyzed the polymer-Ce6 interaction using fluorescence spectroscopy and atomic-force microscopy, and tested the phototoxicity in vitro. Using fluorescence imaging in vivo we found that injection to mice of Ce6 in a formulation with PVA resulted in a higher tumor-to-normal ratio and greater photobleaching when compared with either the use of Ce6 alone, or with the effects of Photodithazine. Tumor growth study and histological examination of CT26 tumors revealed fast, reproducible tumor regression and more advanced necrosis after PDT with Ce6-PVA. The higher photoactivity of the Ce6-PVA complex was confirmed in a model reaction of tryptophan oxidation and in cultured cells. Therefore, encapsulation of Ce6 in PVA represents a promising strategy for further increasing the selectivity and efficacy of PDT. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chlorin e6; Fluorescence imaging in vivo; Murine colon carcinoma; Photodynamic therapy; Photosensitizer; Polyvinyl alcohol |
| المشرفين على المادة: | 0 (Chlorophyllides) 0 (Photosensitizing Agents) 0 (Porphyrins) 0 (Reactive Oxygen Species) 5S2CCF3T1Z (phytochlorin) 8DUH1N11BX (Tryptophan) 9002-89-5 (Polyvinyl Alcohol) |
| تواريخ الأحداث: | Date Created: 20171226 Date Completed: 20180212 Latest Revision: 20211204 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jphotobiol.2017.12.016 |
| PMID: | 29277008 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2682 |
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| DOI: | 10.1016/j.jphotobiol.2017.12.016 |